Mouse pigment epithelium-derived factor,PEDF ELISA Kit

Mouse pigment epithelium-derived factor,PEDF ELISA Kit

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Mfr.No.

CSB-E08820m

Alternative Names

serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1, EPC-1, PEDF, PIG35, pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), m

Reactivity

Mouse

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CD82 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99-110
EDTA plasma (n=4)	89	82-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD82 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	97
	Range %	92-101
1:4	Average %	95
	Range %	90-99
1:8	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.748	2.702	2.725	2.601
5	2.131	2.192	2.162	2.038
2.5	1.491	1.416	1.454	1.330
1.25	0.866	0.827	0.847	0.723
0.625	0.546	0.502	0.524	0.400
0.312	0.363	0.391	0.377	0.253
0.156	0.254	0.239	0.247	0.123
0	0.125	0.123	0.124

Species

Mus musculus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P97298
Antigen	serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

Applications

ELISA

Research Area

SERPINF1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dual Regulation of Adipose Triglyceride Lipase by Pigment Epithelium-Derived Factor: A Novel Mechanistic Insight into Progressive Obesity Dai Z et al,Mol Cell Endocrinol,2013 Jul 10.pii: S0303-7207(13)00284-0. Intracellular Pigment Epithelium-Derived Factor Contributes to Triglyceride Degradation Dai Z et al,Int J Biochem Cell Biol,2013 Jul 22.pii: S1357-2725(13)00231-8. High levels of pigment epithelium-derived factor in diabetes impair wound healing through suppression of Wnt signaling Qi W.et al,Diabetes.,64(4):1407-19

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SKU	Supplier No.	Size	Your price
BHE10506441	CSB-E08820m	96T	$790	Add to Cart
BHE10506441	CSB-E08820m	5x96T	$3792	Add to Cart
BHE10506441	CSB-E08820m	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.