Mouse Hif1alpha(Hypoxia-inducible factor 1-alpha) ELISA Kit

Mfr.No.

EM0310

Alternative Names

Hif1alpha ELISA Kit, HIF-1alpha ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

125-8000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Hif1alpha and the recovery rates were calculated by comparing the measured value to the expected amount of Hif1alpha in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-103	96
EDTA plasma(n=5)	88-100	96
heparin plasma(n=5)	88-101	96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hif1alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-105%	85-103%	90-99%
EDTA plasma(n=5)	87-99%	86-101%	82-95%
heparin plasma(n=5)	81-98%	80-100%	80-98%

Sensitivity

< 75pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Hif1α/HIF-1α/HIF1A/ARNT-interacting protein/Basic-helix-loop-helix-PAS protein MOP1/BHLHE78/Class E basic helix-loop-helix protein 78/HIF-1-alpha/HIF1-alpha/hypoxia-inducible factor 1-alpha/Member of PAS protein 1/member of PAS superfamily 1/PAS domain-containing protein 8

Notes

For Research Use Only

UniProt ID	Q61221
Antigen	Hif1α

Applications	ELISA
Applications Notes	Hif1alpha ELISA Kit allows for the in vitro quantitative determination of Hif1alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: The Faseb Journal Title: HIF-1a stabilization reduces retinal degeneration in a mouse model of retinitis pigmentosa

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SKU	Supplier No.	Size	Your price	Quantity
BHE10805495	EM0310	96T	$400		Add to Cart

Mouse Hif1alpha(Hypoxia-inducible factor 1-alpha) ELISA Kit

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