Mouse ferritin,FE ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08827m

Alternative Names

ferritin,FE

Reactivity

Mouse

Detection Range

37.5 ng/mL-2400 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ATP5G1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5G1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-92
1:2	Average %	94
	Range %	89-98
1:4	Average %	89
	Range %	85-93
1:8	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

9.4 ng/mL

Limit Of Detection

450 nm

Antigen	ferritin,FE

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Adenoviral vector mediated ferritin over-expression in mesenchymal stem cells detected by 7T MRI in vitro Dai HY.et al,PLoS One,Sep 25 Iron Overload Impairs Normal Hematopoietic Stem And Progenitor Cells Through Reactive Oxygen Species And Shortens Survival In Myelodysplastic Syndrome Mice Xin Jin.et al,Haematologica, June

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