Mouse Erythropoietin - EPO ELISA Kit

Mfr.No.

E0003Mo

Sensitivity

2.38pg/ml

Detection Range

5-2000pg/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Erythropoietin (also known as EPO) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.>

Alternative Names

EPO;Erythropoietin

Molecular Weight

21365

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0003Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	150.8	10.73	7.1
Intra-Assay	2	18	801.4	27.02	3.4
Intra-Assay	3	18	325.8	21.66	6.6

Background

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Protocol

These standard curves of E0003Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.164	2.186	2.132
	2.209
600pg/ml	1.388	1.353	1.298
	1.318
300pg/ml	0.749	0.809	0.755
	0.869
150pg/ml	0.516	0.494	0.44
	0.473
75pg/ml	0.324	0.323	0.269
	0.323
0pg/ml	0.058	0.054	0
	0.051

Gene ID	13856
UniProt ID	P07321

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107932	E0003Mo	48T	$320		Add to Cart
BHE12107932	E0003Mo	96T	$458		Add to Cart

Mouse Erythropoietin - EPO ELISA Kit

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