Mouse Caspase-9 ELISA Kit

Mfr.No.

E0627Mo

Alternative Names

APAF-3;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;CASP 9;CASP9;CASP-9;Caspase-9;Caspase-9 subunit p10;Caspase-9 subunit p35;ICE-LAP6;ICE-like apoptotic protease 6

Reactivity

Mouse

Detection Range

7-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

49979

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.29ng/L

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) (By similarity).

Gene ID	12371
UniProt ID	Q8C3Q9
Antigen	CASP9

Research Area

Others

Protocol

These standard curves of E0627Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.326	2.204	2.158
	2.082
400ng/L	1.37	1.385	1.338
	1.399
200ng/L	0.742	0.834	0.788
	0.926
100ng/L	0.466	0.487	0.441
	0.508
50ng/L	0.311	0.274	0.228
	0.237
0ng/L	0.043	0.046	0
	0.05

Precision

We measured random samples of E0627Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	88.5	4.51	5.1
Intra-Assay	2	18	355.1	15.75	4.4
Intra-Assay	3	18	174	10.27	5.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108505	E0627Mo	48T	$320		Add to Cart
BHE12108505	E0627Mo	96T	$458		Add to Cart

Mouse Caspase-9 ELISA Kit

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