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Home / Primary Antibodies / Monoclonal Antibodies

TCP1 alpha Antibody, Clone 91a - SMC-479

BHA11904970
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+1 866.986.9598
Email
[email protected]
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Swissprot P11983
Accession Number NP_038714.2
Gene Id 21454
Target TCP1 alpha
Clone No 91a
Research Area Cancer, Heat Shock, Cell Signaling, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IP, WB, ICC/IF, FCM
Description WB ; ICC ; IF ; IP ; FCM
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11904970-1 SMC-479D Unconjugated 100 ug $306
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BHA11904970-2 SMC-479D-A390 ATTO 390 100 ug $360
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BHA11904970-3 SMC-479D-A488 ATTO 488 100 ug $359
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BHA11904970-4 SMC-479D-A594 ATTO 594 100 ug $359
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BHA11904970-5 SMC-479D-ALP Alkaline Phosphatase 100 ug $352
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BHA11904970-6 SMC-479D-APC APC 100 ug $358
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BHA11904970-7 SMC-479D-BI Biotin 100 ug $354
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BHA11904970-8 SMC-479D-FITC FITC 100 ug $350
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BHA11904970-9 SMC-479D-HRP HRP 100 ug $345
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BHA11904970-10 SMC-479D-PCP PerCP 100 ug $358
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BHA11904970-11 SMC-479D-RPE RPE 100 ug $355
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BHA11904970-12 SMC-479S Unconjugated 12 ug $44
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