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Home / Primary Antibodies / Monoclonal Antibodies

Notch1 Antibody, Clone S253-32 - SMC-430

BHA11904077
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+1 866.986.9598
Email
[email protected]
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Swissprot Q01705
Accession Number NP_032740.3
Gene Id 18128
Target Notch1
Clone No S253-32
Research Area cardiovascular System, Cell Signaling, Epigenetics and Nuclear Signaling, Heart, Neuroscience
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Shipping Temperature Blue Ice or 4°C
Specificity Detects >270kDa. Does not cross-react with Notch 2 or Notch3.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications WB, ICC/IF
Description WB ; ICC ; IF
Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719.

2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.

3. Lindsell C.E., et al. (1995) Cell 80: 909-917.

4. Uyttendaele H., et al. (1996) Development 122: 2251-2259.

5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11904077-1 SMC-430D Unconjugated 100 ug $330
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BHA11904077-2 SMC-430D-A390 ATTO 390 100 ug $384
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BHA11904077-3 SMC-430D-A488 ATTO 488 100 ug $383
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BHA11904077-4 SMC-430D-A594 ATTO 594 100 ug $383
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BHA11904077-5 SMC-430D-ALP Alkaline Phosphatase 100 ug $376
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BHA11904077-6 SMC-430D-APC APC 100 ug $382
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BHA11904077-7 SMC-430D-BI Biotin 100 ug $378
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BHA11904077-8 SMC-430D-FITC FITC 100 ug $374
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BHA11904077-9 SMC-430D-HRP HRP 100 ug $370
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BHA11904077-10 SMC-430D-PCP PerCP 100 ug $382
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BHA11904077-11 SMC-430D-RPE RPE 100 ug $380
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BHA11904077-12 SMC-430S Unconjugated 12 ug $44
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