Mouse Anti-N-terminal Arginylation Monoclonal IgG1

Mfr.No.	SMC-263, SMC-263D, SMC-263D-A390, SMC-263D-A488, SMC-263D-A594, SMC-263D-ALP, SMC-263D-APC, SMC-263D-BI, SMC-263D-FITC, SMC-263D-HRP, SMC-263D-PCP, SMC-263D-RPE, SMC-263S
Alternative Names	N-terminal Arginine Antibody, N-terminal Arginylation Antibody, N-terminal Arginylated Antibody, N terminal Arginine Antibody, N terminal Arginylation Antibody, N terminal Arginylated Antibody, Amino-terminal Arginine Antibody, Amino-terminal Arginylation Antibody, Amino-terminal Arginylated Antibody, Amino terminal Arginine Antibody, Amino terminal Arginylation Antibody, Amino terminal Arginylated Antibody
Antigen	N-terminal Arginylation
Categories	Antibodies
Cellular Localization	Endoplasmic Reticulum
Certificate Of Analysis	A 1:1000 dilution of SMC-263 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-N-terminal Arginylation Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic N-terminal arginylated peptide conjugated to KLH
Immunogen Region	Synthetic N-terminal arginylated peptide conjugated to KLH
Isotype	IgG1
Launch Date	2017/02/23
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	N-terminal Arginylation
Clone No	4A9
Research Area	Cell Signaling, Post-translational Modifications, Arginylation

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); FCM (1:50); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	FACS, WB, FCM
Description	WB ; FCM ; FACS
Dilution	WB (1:1000); FCM (1:50); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11902310-1	SMC-263D	Unconjugated	100 ug	$432	Add to Cart
BHA11902310-2	SMC-263D-A390	ATTO 390	100 ug	$486	Add to Cart
BHA11902310-3	SMC-263D-A488	ATTO 488	100 ug	$485	Add to Cart
BHA11902310-4	SMC-263D-A594	ATTO 594	100 ug	$485	Add to Cart
BHA11902310-5	SMC-263D-ALP	Alkaline Phosphatase	100 ug	$479	Add to Cart
BHA11902310-6	SMC-263D-APC	APC	100 ug	$484	Add to Cart
BHA11902310-7	SMC-263D-BI	Biotin	100 ug	$481	Add to Cart
BHA11902310-8	SMC-263D-FITC	FITC	100 ug	$475	Add to Cart
BHA11902310-9	SMC-263D-HRP	HRP	100 ug	$471	Add to Cart
BHA11902310-10	SMC-263D-PCP	PerCP	100 ug	$484	Add to Cart
BHA11902310-11	SMC-263D-RPE	RPE	100 ug	$482	Add to Cart
BHA11902310-12	SMC-263S	Unconjugated	12 ug	$44	Add to Cart

N-terminal Arginylation Antibody, Clone 4A9 - SMC-263

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