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Home / Primary Antibodies / Monoclonal Antibodies

LAMP1 Antibody, Clone Ly1C6 - SMC-140

BHA11900580
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Email
[email protected]
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Swissprot P14562
Accession Number NP_036989.1
Gene Id 25328
Target LAMP1
Clone No Ly1C6
Research Area Cell Signaling, Tags and Cell Markers, Organelle Markers, Chaperone Proteins, Neuroscience, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3).
This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~120kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IP, WB, ICC/IF
Description WB ; ICC ; IF ; IP
Dilution WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68.

2. Furuta, K. et al. (1999) EMBO J. 17(5):1304-14.

3. Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76.

4. Kannan, K., et al. (1996) Cell Immunol. 171: 10-19.

5. Lewis, V., et al. (1985) J. Cell Biol. 100: 1839-1847.

6. Jones, K.A., et al. (2004) Exp Cell Res 295(2): 512-524.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900580-1 SMC-140D Unconjugated 100 ug $320
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BHA11900580-2 SMC-140C Unconjugated 25 ug $179
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BHA11900580-3 SMC-140D-A390 ATTO 390 100 ug $374
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BHA11900580-4 SMC-140D-A488 ATTO 488 100 ug $373
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BHA11900580-5 SMC-140D-A594 ATTO 594 100 ug $373
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BHA11900580-6 SMC-140D-ALP Alkaline Phosphatase 100 ug $366
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BHA11900580-7 SMC-140D-APC APC 100 ug $372
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BHA11900580-8 SMC-140D-BI Biotin 100 ug $369
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BHA11900580-9 SMC-140D-FITC FITC 100 ug $363
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BHA11900580-10 SMC-140D-HRP HRP 100 ug $359
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BHA11900580-11 SMC-140D-PCP PerCP 100 ug $372
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BHA11900580-12 SMC-140D-RPE RPE 100 ug $370
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BHA11900580-13 SMC-140S Unconjugated 12 ug $44
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