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Home / Primary Antibodies / Monoclonal Antibodies

HSF1 Antibody, Clone 10H8 - SMC-118

BHA11900261
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Email
[email protected]
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Swissprot P38532
Accession Number NP_032322.1
Gene Id 15499
Target HSF1
Clone No 10H8
Research Area Cancer, Heat Shock, Cell Signaling, Epigenetics and Nuclear Signaling, Cardiovascular System, Heart
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, GS, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA ; GS
Dilution WB (1:1000), IHC (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934.

2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82.

3.Tanaka KI et al. (2007). JBC Papers Online Manuscript M704081200.

4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796.

5. McMillan D. R., Xiao X., Shao L., Graves K., and Benjamin I. J. (1998) J Bio Chem 273: 7523-7528.

6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774.

7. Fiorenza M.T., Farkas T., Dissing M., Kolding D. and Zimarino V. (1995) Nucleic Acids Res. 23 (3):467-474.

8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293.

9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397.

10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334- 1343.

11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900261-1 SMC-118D Unconjugated 100 ug $254
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BHA11900261-2 SMC-118C Unconjugated 25 ug $114
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BHA11900261-3 SMC-118D-A390 ATTO 390 100 ug $307
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BHA11900261-4 SMC-118D-A488 ATTO 488 100 ug $306
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BHA11900261-5 SMC-118D-A594 ATTO 594 100 ug $306
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BHA11900261-6 SMC-118D-ALP Alkaline Phosphatase 100 ug $299
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BHA11900261-7 SMC-118D-APC APC 100 ug $305
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BHA11900261-8 SMC-118D-BI Biotin 100 ug $301
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BHA11900261-9 SMC-118D-FITC FITC 100 ug $297
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BHA11900261-10 SMC-118D-HRP HRP 100 ug $293
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BHA11900261-11 SMC-118D-PCP PerCP 100 ug $305
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BHA11900261-12 SMC-118D-RPE RPE 100 ug $303
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BHA11900261-13 SMC-118S Unconjugated 12 ug $44
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