This recombinant His Tag antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Formulation
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this recombinant His Tag antibody.
Isotype
IgG
Predicted Reactivity
All Species
Reactivity
All Species
Recombinant
Yes
Clone No
RM146
Concentration
1 mg/ml
Format
Purified
Purification
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant His Tag antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Applications
FACS, WB, IP, ICC, IHC
Dilution
Western blot: 0.1-1ug/ml,Immunoprecipitation: 0.5-2ug/mg,Immunocytochemistry: 0.5-2ug/ml,Flow cytometry: 0.5-2ug/ml,Immunohistochemistry: 0.1-1ug/ml (1)