Mouse Anti-HeXanoyl-Lysine adduct (HEL) Monoclonal IgG1

Mfr.No.	SMC-508, SMC-508D, SMC-508D-A390, SMC-508D-A488, SMC-508D-A594, SMC-508D-ALP, SMC-508D-APC, SMC-508D-BI, SMC-508D-FITC, SMC-508D-HRP, SMC-508D-PCP, SMC-508D-RPE, SMC-508S
Alternative Names	Hexanoyl-Lysine adduct Antibody, HEL (Hexanoyl-Lysine adduct) Antibody, HEL Antibody, HEL Adduct Antibody, Hexanoyl-Lys adduct Antibody, Hexanoyl-Lys Antibody, Hexanoyl-Lysine (HEL) adduct Antibody, Hexanoyl-Lys (HEL) Antibody, Hexanoyl Lysine adduct Antibody, Hexanoyl-Lysine adduct-modified protein Antibody, Hexanoyl-Lysine Adduct (HEL) Antibody
Antigen	Hexanoyl-Lysine adduct
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-508 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 ug of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Region	Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
Isotype	IgG1
Launch Date	2017/03/23
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	Hexanoyl-Lysine adduct
Clone No	5D9
Research Area	Cancer, Oxidative Stress, Lipid peroxidation

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C
Trademark	MOLECULAR SIGNATURE

Applications	ELISA, FACS, WB, ICC/IF, FCM, IHC
Description	WB ; ICC ; IF ; FACS ; FCM ; ELISA
Dilution	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); IHC (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Sakai, K et al. (2014) Subcell. Biochem. 77:61-72.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11905445-1	SMC-508D	Unconjugated	100 ug	$409	Add to Cart
BHA11905445-2	SMC-508D-A390	ATTO 390	100 ug	$463	Add to Cart
BHA11905445-3	SMC-508D-A488	ATTO 488	100 ug	$462	Add to Cart
BHA11905445-4	SMC-508D-A594	ATTO 594	100 ug	$462	Add to Cart
BHA11905445-5	SMC-508D-ALP	Alkaline Phosphatase	100 ug	$455	Add to Cart
BHA11905445-6	SMC-508D-APC	APC	100 ug	$461	Add to Cart
BHA11905445-7	SMC-508D-BI	Biotin	100 ug	$458	Add to Cart
BHA11905445-8	SMC-508D-FITC	FITC	100 ug	$453	Add to Cart
BHA11905445-9	SMC-508D-HRP	HRP	100 ug	$449	Add to Cart
BHA11905445-10	SMC-508D-PCP	PerCP	100 ug	$461	Add to Cart
BHA11905445-11	SMC-508D-RPE	RPE	100 ug	$459	Add to Cart
BHA11905445-12	SMC-508S	Unconjugated	12 ug	$44	Add to Cart

Hexanoyl-Lysine adduct Antibody, Clone 5D9 - SMC-508