Mouse Anti-HA tag Monoclonal IgG3

Mfr.No.	SMC-260, SMC-260D, SMC-260D-A390, SMC-260D-A488, SMC-260D-A594, SMC-260D-BI, SMC-260D-FITC
Immunogen Species	Human
Alternative Names	HA epitope tag antibody, HA1 antibody, HA2 antibody, hemagglutinin antibody, Hemagglutinin HA1 chain antibody, Hemagglutinin HA2 chain antibody
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-260 was sufficient for detection of HA Tag in 10 ug of Human Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-HA tag Monoclonal IgG3
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH., Homo Sapiens (Human)
Immunogen Region	A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
Isotype	IgG3
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Species Independent

Target	HA tag
Clone No	HA.C5
Research Area	Tags and Cell Markers, Epitope Tags, HA Tag

Buffer	PBS, pH 7.4, 50% glyercol, 0.05% sodium azide
Dilutions	WB (1:1000), ICC/IF (1:400), IP (1:400); optimal dilutions for assays should be determined by the user.
Scientific Background	Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 and has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Shipping Temperature	Blue Ice or 4°C
Specificity	HA-tag monoclonal antibody. Recognizes HA-tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Storage	-20ºC
Storage Buffer	PBS, pH 7.4, 50% glyercol, 0.05% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IP, WB, ICC/IF
Description	WB ; ICC ; IF ; IP
Dilution	WB (1:1000), ICC/IF (1:400), IP (1:400); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Schembri, L. et al. (2007) Nature Methods 4 (2): 107108.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11902255-1	SMC-260D	Unconjugated	100 ul	$286	Add to Cart
BHA11902255-2	SMC-260D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11902255-3	SMC-260D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11902255-4	SMC-260D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11902255-5	SMC-260D-BI	Biotin	100 ul	$334	Add to Cart
BHA11902255-6	SMC-260D-FITC	FITC	100 ul	$330	Add to Cart

HA tag Antibody, Clone HA.C5 - SMC-260