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Home / Primary Antibodies / Monoclonal Antibodies

GRP94 Antibody, Clone 6B12 - SMC-541

BHA11905939
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+1 866.986.9598
Email
[email protected]
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Swissprot P14625
Accession Number NP_003290.1
Gene Id 7184
Target GRP94
Clone No 6B12
Research Area Cancer, Heat Shock, Cell Signaling, ER Proteins, Tags and Cell Markers, Organelle Markers, Cancer Metabolism, Response to Hypoxia, Metabolism, Metabolism processes, Hypoxia, Protein Trafficking
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 1 mg/ml
Dilutions WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5).
Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
Shipping Temperature Blue Ice or 4°C
Specificity Detects 94 kDa band corresponding to GRP94.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications WB
Description WB
Dilution WB (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128.

2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-98.

3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262: 8875-8883.

4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9): 5643-5649.

5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-48338.

6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269.

7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637.

8. Allen S. et al. (2000) Blood 96(2): 560-568.

9. Sato K et al. (2001) Blood 98(6): 1852-1857.

10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5): 371-378.

11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858.

12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386.

13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104.

14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-24900.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11905939-1 SMC-541D Unconjugated 100 ug $330
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BHA11905939-2 SMC-541D-A390 ATTO 390 100 ug $384
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BHA11905939-3 SMC-541D-A488 ATTO 488 100 ug $383
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BHA11905939-4 SMC-541D-A594 ATTO 594 100 ug $383
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BHA11905939-5 SMC-541D-ALP Alkaline Phosphatase 100 ug $376
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BHA11905939-6 SMC-541D-APC APC 100 ug $382
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BHA11905939-7 SMC-541D-BI Biotin 100 ug $378
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BHA11905939-8 SMC-541D-FITC FITC 100 ug $374
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BHA11905939-9 SMC-541D-HRP HRP 100 ug $370
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BHA11905939-10 SMC-541D-PCP PerCP 100 ug $382
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BHA11905939-11 SMC-541D-RPE RPE 100 ug $380
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BHA11905939-12 SMC-541S Unconjugated 12 ug $44
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