GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein;
Categories
Primary Antibodies
Clonality
monoclonal
Description
The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.
Host
Mouse
Immunogen
Purified recombinant EYFP.tag full length expressed in E.coli.
Isotype
IgG1
Raised In
Mouse
Reactivity
Bacillus Cereus
Regulatory
RUO
Relevance
The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.
Species
Bacillus cereus
Specificity
Transfected
Buffer
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Description
Transfected
Form
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Affinity purified
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.