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Home / Primary Antibodies / Monoclonal Antibodies

CaMKII Antibody, Clone 22B1 - SMC-125

BHA11900380
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Email
[email protected]
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Swissprot P11275
Accession Number NP_037052.1
Gene Id 25400
Target CaMKII
Clone No 22B1
Research Area Cell Signaling, Post-translational Modifications, Phosphorylation
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Shipping Temperature Blue Ice or 4°C
Specificity Detects phosphorylated CaMKII from rat tissues. This antibody is specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution WB (1:1000), IHC (1:100), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013.

2. Barria A. et al. (1997) Science 276: 2042–2045.

3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798.

4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.

5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.

6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.

7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.

8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12.

9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.

10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.

11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.

12. Shen K. and Meyer Y. (1999) Science 284: 162-167.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900380-1 SMC-125D Unconjugated 100 ug $306
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BHA11900380-2 SMC-125C Unconjugated 25 ug $150
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BHA11900380-3 SMC-125D-A390 ATTO 390 100 ug $360
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BHA11900380-4 SMC-125D-A488 ATTO 488 100 ug $359
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BHA11900380-5 SMC-125D-A594 ATTO 594 100 ug $359
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BHA11900380-6 SMC-125D-ALP Alkaline Phosphatase 100 ug $352
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BHA11900380-7 SMC-125D-APC APC 100 ug $358
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BHA11900380-8 SMC-125D-BI Biotin 100 ug $354
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BHA11900380-9 SMC-125D-FITC FITC 100 ug $350
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BHA11900380-10 SMC-125D-HRP HRP 100 ug $345
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BHA11900380-11 SMC-125D-PCP PerCP 100 ug $358
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BHA11900380-12 SMC-125D-RPE RPE 100 ug $355
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BHA11900380-13 SMC-125S Unconjugated 12 ug $44
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