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Home / Primary Antibodies / Monoclonal Antibodies

Anti-U2AF65/U2AF2 Picoband® Antibody (monoclonal, 10F4)

SKU : BHA21003154

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Mfr.No.	M03639, M03639-10ug, M03639-Biotin, M03639-Cy3, M03639-Dylight488, M03639-Dylight550, M03639-Dylight594, M03639-FITC, M03639-HRP, M03639-APC, M03639-PE, M03639-iFluor647, M03639-carrier-free
Alternative Names	eukaryotic translation initiation factor 6
Background	Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-U2AF65/U2AF2 Picoband® Antibody (monoclonal, 10F4) catalog # M03639. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved
Host	Mouse
Immunogen Region	E.coli-derived human U2AF65/U2AF2 recombinant protein (Position: M238-H470).
Isotype	Mouse IgG2b
Molecular Weight	65 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Eukaryotic translation initiation factor 6; eIF-6; B (2)GCN homolog; B4 integrin interactor; CAB; p27 (BBP); EIF6; EIF3A; ITGB4BP; OK/SW-cl.27
Tissue Specificity	Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	P26368
Gene Id	11338
Gene Name	U2AF2
Clone No	Clone: 10F4
Subcellular Location	nucleolus. Cytoplasm

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Kielkopf, C. L., Rodionova, N. A., Green, M. R., Burley, S. K. A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer. Cell 106: 595-605, 2001.<br /><br />2. Mackereth, C. D., Madl, T., Bonnal, S., Simon, B., Zanier, K., Gasch, A., Rybin, V., Valcarcel, J., Sattler, M. Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF. Nature 475: 408-411, 2011.<br /><br />3. Sickmier, E. A., Frato, K. E., Shen, H., Paranawithana, S. R., Green, M. R., Kielkopf, C. L. Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. Molec. Cell 23: 49-59, 2006.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003154-1	M03639	-	100 ug	$370		Add to Cart
BHA21003154-2	M03639-10ug	Sample	10 ug	$99		Add to Cart
BHA21003154-3	M03639-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003154-4	M03639-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003154-5	M03639-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003154-6	M03639-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003154-7	M03639-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003154-8	M03639-FITC	FITC	100 ug	$570		Add to Cart
BHA21003154-9	M03639-HRP	HRP	100 ug	$570		Add to Cart
BHA21003154-10	M03639-APC	APC	100 ug	$670		Add to Cart
BHA21003154-11	M03639-PE	PE	100 ug	$670		Add to Cart
BHA21003154-12	M03639-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003154-13	M03639-carrier-free	carrier-free	100 ug	$370		Add to Cart

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