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Home / Primary Antibodies / Monoclonal Antibodies

Anti-Transketolase/TKT Picoband® Antibody (monoclonal, 3E5)

SKU : BHA21003151

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Mfr.No.	M02197, M02197-10ug, M02197-Biotin, M02197-Cy3, M02197-Dylight488, M02197-Dylight550, M02197-Dylight594, M02197-FITC, M02197-HRP, M02197-APC, M02197-PE, M02197-iFluor647, M02197-carrier-free
Alternative Names	adenosine kinase
Background	Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is ly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Transketolase/TKT Picoband® Antibody (monoclonal, 3E5) catalog # M02197. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Host	Mouse
Immunogen Region	E.coli-derived human Transketolase/TKT recombinant protein (Position: M1-A116).
Isotype	Mouse IgG2a
Molecular Weight	68 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Adenosine kinase; AK; Adenosine 5'-phosphotransferase; ADK
Tissue Specificity	Widely expressed. Highest level in placenta, liver, muscle and kidney.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	P29401
Gene Id	7086
Gene Name	TKT
Clone No	Clone: 3E5
Subcellular Location	Nucleus. Cytoplasm.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 1-2ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence; 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Abedinia, M., Layfield, R., Jones, S. M., Nixon, P. F., Mattick, J. S. Nucleotide and predicted amino acid sequence of a cDNA clone encoding part of human transketolase. Biochem. Biophys. Res. Commun. 183: 1159-1166, 1992.<br /><br />2. Boyle, L., Wamelink, M. M. C., Salomons, G. S., Roos, B., Pop, A., Dauber, A., Hwa, V., Andrew, M., Douglas, J., Feingold, M., Kramer, N., Saitta, S., Retterer, K., Cho, M. T., Begtrup, A., Monaghan, K. G., Wynn, J., Chung, W. K. Mutations in TKT are the cause of a syndrome, including short stature, developmental delay, and congenital heart defects. Am. J. Hum. Genet. 98: 1235-1242, 2016.<br /><br />3. Coy, J. F., Dubel, S., Kioschis, P., Thomas, K., Micklem, G., Delius, H., Poustka, A. Molecular cloning of tissue-specific transcripts of a transketolase-related gene: implications for the evolution of new vertebrate genes. Genomics 32: 309-316, 1996.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003151-1	M02197	-	100 ug	$370		Add to Cart
BHA21003151-2	M02197-10ug	Sample	10 ug	$99		Add to Cart
BHA21003151-3	M02197-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003151-4	M02197-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003151-5	M02197-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003151-6	M02197-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003151-7	M02197-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003151-8	M02197-FITC	FITC	100 ug	$570		Add to Cart
BHA21003151-9	M02197-HRP	HRP	100 ug	$570		Add to Cart
BHA21003151-10	M02197-APC	APC	100 ug	$670		Add to Cart
BHA21003151-11	M02197-PE	PE	100 ug	$670		Add to Cart
BHA21003151-12	M02197-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003151-13	M02197-carrier-free	carrier-free	100 ug	$370		Add to Cart

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