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Home / Primary Antibodies / Monoclonal Antibodies

Anti-RAB27A Antibody Picoband® (monoclonal, 2F5)

SKU : BHA21002273

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Mfr.No.	M01608, M01608-10ug, M01608-Biotin, M01608-Cy3, M01608-Dylight488, M01608-Dylight550, M01608-Dylight594, M01608-FITC, M01608-HRP, M01608-APC, M01608-PE, M01608-iFluor647, M01608-carrier-free
Alternative Names	RAB27A, member RAS oncogene family
Background	Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alteative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-RAB27A Antibody Picoband® (monoclonal, 2F5) catalog # M01608. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Host	Mouse
Immunogen Region	E. coli-derived human RAB27A recombinant protein (Position: L98-K216).
Isotype	Mouse IgG2b
Molecular Weight	27 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A; RAB27
Tissue Specificity	Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	P51159
Gene Id	5873
Gene Name	RAB27A
Clone No	Clone: 2F5
Subcellular Location	Lysosome. Late endosome. Membrane. Lipid-anchor. Melanosome.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml<br> Immunocytochemistry/Immunofluorescence, 2ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells<br>

Reference	1. Aksu, G., Kutukculer, N., Genel, F., Vergin, C., Omowaire, B. Griscelli syndrome without hemophagocytosis in an eleven-year-old girl: expanding the phenotypic spectrum ofRab27A mutations in humans. Am. J. Med. Genet. 116A: 329-333, 2003.<br /><br />2. Kuroda, T. S., Fukuda, M., Ariga, H., Mikoshiba, K. The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem. 277: 9212-9218, 2002.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002273-1	M01608	-	100 ug	$370		Add to Cart
BHA21002273-2	M01608-10ug	Sample	10 ug	$99		Add to Cart
BHA21002273-3	M01608-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002273-4	M01608-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002273-5	M01608-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002273-6	M01608-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002273-7	M01608-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002273-8	M01608-FITC	FITC	100 ug	$570		Add to Cart
BHA21002273-9	M01608-HRP	HRP	100 ug	$570		Add to Cart
BHA21002273-10	M01608-APC	APC	100 ug	$670		Add to Cart
BHA21002273-11	M01608-PE	PE	100 ug	$670		Add to Cart
BHA21002273-12	M01608-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002273-13	M01608-carrier-free	carrier-free	100 ug	$370		Add to Cart

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