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Home / Primary Antibodies / Monoclonal Antibodies

Anti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7)

SKU : BHA21004088

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Mfr.No.	M02425, M02425-10ug, M02425-Biotin, M02425-Cy3, M02425-Dylight488, M02425-Dylight550, M02425-Dylight594, M02425-FITC, M02425-HRP, M02425-APC, M02425-PE, M02425-iFluor647, M02425-carrier-free
Alternative Names	peptidylprolyl isomerase D
Background	Poly(rC)-binding protein 2 is a protein that in humans is encoded by the PCBP2 gene. The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7) catalog # M02425. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different c
Host	Mouse
Immunogen Region	E.coli-derived human PCBP2/hnRNP E2 recombinant protein (Position: Q197-K276).
Isotype	Mouse IgG2a
Molecular Weight	39 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Peptidyl-prolyl cis-trans isomerase D; PPIase D
Tissue Specificity	Widely expressed.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	Q15366
Gene Id	5094
Gene Name	PCBP2
Clone No	Clone: 4B9C7
Subcellular Location	Cytoplasm

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB, ICC, IF, Flow Cytometry
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human<br> Flow Cytometry (Fixed), 1-3 ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Bejerano, G., Lowe, C. B., Ahituv, N., King, B., Siepel, A., Salama, S. R., Rubin, E. M., Kent, W. J., Haussler, D. A distal enhancer and an ultraconserved exon are derived from a novel retroposon. Nature 441: 87-90, 2006. <br /><br />2. Chkheidze, A. N., Liebhaber, S. A. A novel set of nuclear localization signals determine distributions of the alpha-CP RNA-binding proteins. Molec. Cell. Biol. 23: 8405-8415, 2003.<br /><br />3. Eiring, A. M., Harb, J. G., Neviani, P., Garton, C., Oaks, J. J., Spizzo, R., Liu, S., Schwind, S., Santhanam, R., Hickey, C. J., Becker, H., Chandler, J. C., and 13 others. miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts. Cell 140: 652-665, 2010.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21004088-1	M02425	-	100 ug	$370		Add to Cart
BHA21004088-2	M02425-10ug	Sample	10 ug	$99		Add to Cart
BHA21004088-3	M02425-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21004088-4	M02425-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21004088-5	M02425-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21004088-6	M02425-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21004088-7	M02425-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21004088-8	M02425-FITC	FITC	100 ug	$570		Add to Cart
BHA21004088-9	M02425-HRP	HRP	100 ug	$570		Add to Cart
BHA21004088-10	M02425-APC	APC	100 ug	$670		Add to Cart
BHA21004088-11	M02425-PE	PE	100 ug	$670		Add to Cart
BHA21004088-12	M02425-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21004088-13	M02425-carrier-free	carrier-free	100 ug	$370		Add to Cart

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