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Home / Primary Antibodies / Monoclonal Antibodies

Anti-p95 NBS1 Antibody Picoband® (monoclonal, 5D7A1)

SKU : BHA21003755

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Mfr.No.	M00732-1, M00732-1-10ug, M00732-1-Biotin, M00732-1-Cy3, M00732-1-Dylight488, M00732-1-Dylight550, M00732-1-Dylight594, M00732-1-FITC, M00732-1-HRP, M00732-1-APC, M00732-1-PE, M00732-1-iFluor647, M00732-1-carrier-free
Alternative Names	BH3 interacting domain death agonist
Background	p95 NBS1, also known as NBN or Nibrin, is a protein which in humans is encoded by the NBN gene. Nibrin is a protein associated with the repair of double strand breaks (DSBs) which pose serious damage to a genome. It is a 754 amino acid protein identified as a member of the NBS1/hMre11/RAD50(N/M/R, more commonly referred to asMRN) double strand DNA break repair complex. This complex recognizes DNA damage and rapidly relocates to DSB sites and forms nuclear foci. It also has a role in regulation of N/M/R (MRN) protein complex activity which includes end-processing of both physiological and mutagenic DNA double strand breaks (DSBs).
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-p95 NBS1 Antibody Picoband® (monoclonal, 5D7A1) catalog # M00732-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	The major proteolytic product p15 BID allows the release of cytochrome c. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Host	Mouse
Immunogen Region	A synthetic peptide corresponding to a sequence at the C-terminus of human p95 NBS1, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.
Isotype	Mouse IgG2a
Molecular Weight	95 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Apoptic death agonist antibody; Apoptotic death agonist antibody; Apoptotic death agonist BID antibody; BH3 interacting domain death agonist antibody; BH3 interacting domain death agonist p11 antibody; BH3 interacting domain death agonist p13 antibody; BH3 interacting domain death agonist p15 antibody; BH3-interacting domain death agonist p11 antibody; BID antibody; BID isoform ES (1b) antibody; BID isoform L (2) antibody; BID isoform Si6 antibody; BID_HUMAN antibody; Desmocollin type 4 antibody; FP497 antibody; Human BID coding sequence antibody; MGC15319 antibody; MGC42355 antibody; p11 BID antibody; p13 BID antibody; p15 BID antibody; p22 BID antibody
Tissue Specificity	Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	O60934
Gene Id	4683
Gene Name	NBN
Clone No	Clone: 5D7A1
Subcellular Location	Mitochondrion membrane. Mitochondrion outer membrane. Cytoplasm.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB, IHC, ICC, IF, Flow Cytometry
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 ug/ml, Human Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human Flow Cytometry (Fixed), 1-3 ug/1x⁶ cells, Human

Reference	1. "Atlas of Genetics and Cytogenetics in Oncology and Haematology - NBS1". 2. Caey JP, Maser RS, Olivares H, Davis EM, Le Beau M, Yates JR, Hays L, Morgan WF, Petrini JH (May 1998). "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response". Cell 93 (3): 47786. 3. Varon R, Vissinga C, Platzer M, Cerosaletti KM, Chrzanowska KH, Saar K, Beckmann G, Seemanová E, Cooper PR, Nowak NJ, Stumm M, Weemaes CM, Gatti RA, Wilson RK, Digweed M, Rosenthal A, Sperling K, Concannon P, Reis A (May 1998). "Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome". Cell 93 (3): 46776.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003755-1	M00732-1	-	100 ug	$370		Add to Cart
BHA21003755-2	M00732-1-10ug	Sample	10 ug	$99		Add to Cart
BHA21003755-3	M00732-1-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003755-4	M00732-1-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003755-5	M00732-1-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003755-6	M00732-1-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003755-7	M00732-1-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003755-8	M00732-1-FITC	FITC	100 ug	$570		Add to Cart
BHA21003755-9	M00732-1-HRP	HRP	100 ug	$570		Add to Cart
BHA21003755-10	M00732-1-APC	APC	100 ug	$670		Add to Cart
BHA21003755-11	M00732-1-PE	PE	100 ug	$670		Add to Cart
BHA21003755-12	M00732-1-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003755-13	M00732-1-carrier-free	carrier-free	100 ug	$370		Add to Cart

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