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Home / Primary Antibodies / Monoclonal Antibodies

Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2)

SKU : BHA21001965

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Mfr.No.	M03515, M03515-10ug, M03515-Biotin, M03515-Cy3, M03515-Dylight488, M03515-Dylight550, M03515-Dylight594, M03515-FITC, M03515-HRP, M03515-APC, M03515-PE, M03515-iFluor647, M03515-carrier-free
Alternative Names	non-POU domain containing, octamer-binding
Background	Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alteatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) catalog # M03515. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably a
Host	Mouse
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb, identical to the related mouse and rat sequences.
Isotype	Mouse IgG1
Molecular Weight	60 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Non-POU domain-containing octamer-binding protein; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; NMT55; p54 (nrb); p54nrb; NONO; NRB54
Tissue Specificity	Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	Q15233
Gene Id	4841
Gene Name	NONO
Clone No	Clone: 11E2
Subcellular Location	Nucleus. Nucleus, nucleolus. Nucleus speckle. Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunocytochemistry, 0.5-1ug/ml<br> Immunocytochemistry/Immunofluorescence, 5ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells<br>

Reference	1. "Entrez Gene: NONO Non-POU domain containing, octamer-binding".<br /><br />2. Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6". Nucleic Acids Res. 21 (17): 408592.<br /><br />3. Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). "Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer". Diagn. Mol. Pathol. 6(4): 20921.

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Combination SKU	Supplier No.	Size	Conjugation	Your price	Quantity
BHA21001965-1	M03515	100 ug	-	$370		Add to Cart
BHA21001965-2	M03515-10ug	10 ug	Sample	$99		Add to Cart
BHA21001965-3	M03515-Biotin	100 ug	Biotin	$570		Add to Cart
BHA21001965-4	M03515-Cy3	100 ug	Cy3	$570		Add to Cart
BHA21001965-5	M03515-Dylight488	100 ug	Dylight488	$570		Add to Cart
BHA21001965-6	M03515-Dylight550	100 ug	Dylight550	$570		Add to Cart
BHA21001965-7	M03515-Dylight594	100 ug	Dylight594	$570		Add to Cart
BHA21001965-8	M03515-FITC	100 ug	FITC	$570		Add to Cart
BHA21001965-9	M03515-HRP	100 ug	HRP	$570		Add to Cart
BHA21001965-10	M03515-APC	100 ug	APC	$670		Add to Cart
BHA21001965-11	M03515-PE	100 ug	PE	$670		Add to Cart
BHA21001965-12	M03515-iFluor647	100 ug	iFluor647	$670		Add to Cart
BHA21001965-13	M03515-carrier-free	100 ug	carrier-free	$370		Add to Cart

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