This purified recombinant monoclonal antibody is a human anti-IL23alpha that selectively binds to the p19 subunit of heterodimeric IL-23, which is composed of p19 and IL-12 p40. The variable regions for this antibody are based on an FDA-approved drug Skyrizi (risankizumab-rzaa), sold by AbbVie. This antibody has been tested for specific binding affinity to IL23alpha/IL12beta antigen.
assay conditions: Functional validation: The antibody was validated by measuring anti-IL23alpha binding to IL23alpha/IL12beta antigen (Acro Biosystems #ILB-H52W5) in ELISA assay. The IL23alpha/IL12beta protein was coated onto a 96-well plate overnight at 4°C (50 ul/well at a concentration of 6 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified anti-IL23alpha antibody (BPS Bioscience #101453), serially diluted in Blocking Buffer 2, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host
HEK293
Instructions for use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.