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Home / Primary Antibodies / Monoclonal Antibodies

anti-human CD28

SKU : BHA14700045

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Ancell Corporation

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Mfr.No.	177, 177-020, 177-030, 177-040, 177-050, 177-520, 177-820
Alternative Names	Tp44, T44
Background	Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28 identified as CD80(B7-1) and CD86(B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60 kd and 80 kd respectively. Anti-CD28 antibodies have recently been shown to have a strong adjuvant effect when conjugated to the immunogen (4). Antibody ANC28.1 recognizes CD28 expressed on human T cells and also on BHK cells transfected with human CD28. Monomeric and cross linked ANC28.1 will stimulate expression of IL-2 from CD28+ cells.
Formulation	50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
Host	Mouse
Isotype	IgG1k
Reactivity	Human
Specificity	Antibody ANC28.1 recognizes CD28 expressed on T cells and also on BHK cells transfected with human CD28. This antibody has been characterized as a superagonist (5)

Purity	Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Storage	Store at 2 - 5°C. Freeze/Thawing is not recommended.
Storage Buffer	50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).

Applications	EIA, Agonist, FC

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA14700045-1	177-020	Pur WA	100 ug	$235		Add to Cart
BHA14700045-2	177-030	Biotin	100 ug	$325		Add to Cart
BHA14700045-3	177-040	FITC	120 tests	$325		Add to Cart
BHA14700045-4	177-050	PE	120 tests	$350		Add to Cart
BHA14700045-5	177-520	F(ab')2	100 ug	$745		Add to Cart
BHA14700045-6	177-820	Pur/PF	100 ug	$235		Add to Cart

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