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Home / Primary Antibodies / Monoclonal Antibodies

Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband® (monoclonal, 2B12)

SKU : BHA21002717

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Mfr.No.	M09982, M09982-10ug, M09982-Biotin, M09982-Cy3, M09982-Dylight488, M09982-Dylight550, M09982-Dylight594, M09982-FITC, M09982-HRP, M09982-APC, M09982-PE, M09982-iFluor647, M09982-carrier-free
Alternative Names	ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d
Background	Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) also known as AU-rich element RNA-binding protein 1 (AUF1) is a protein that in humans is encoded by the HNRNPD gene. It is mapped to 4q21.22. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alteative splicing of this gene results in four transcript variants.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband® (monoclonal, 2B12) catalog # M09982. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Mitochondrial membrane ATP synthase (F (1)F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist o
Host	Mouse
Immunogen Region	E.coli-derived human hnRNP D/AUF1/HNRNPD recombinant protein (Position: E88-N246).
Isotype	Mouse IgG2a
Molecular Weight	43-45 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	ATP synthase D chain mitochondrial antibody\|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody\|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody\|ATP synthase subunit d antibody\|ATP synthase subunit d, mitochondrial antibody\|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody\|ATP5H antibody\|ATP5H_HUMAN antibody\|ATP5JD antibody\|ATPase subunit d antibody\|ATPQ antibody\|mitochondrial antibody\|My032 protein antibody
Tissue Specificity	Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Uniprot	Q14103
Gene Id	3184
Gene Name	HNRNPD
Clone No	Clone: 2B12
Subcellular Location	Mitochondrion. Mitochondrion inner membrane.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry
Description	Weste blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat<br>Immunocytochemistry/Immunofluorescence, 4ug/ml, Human<br>Immunofluorescence, 4ug/ml, Human<br>Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human

Reference	1. Arao Y, Kuriyama R, Kayama F, Kato S (August 2000). "A nuclear matrix-associated factor, SAF-B, interacts with specific isoforms of AUF1/hnRNP D". Arch. Biochem. Biophys. 380 (2): 22836. <br /><br />2. Sinsimer KS, Gratacós FM, Knapinska AM, Lu J, Krause CD, Wierzbowski AV, Maher LR, Scrudato S, Rivera YM, Gupta S, Turrin DK, De La Cruz MP, Pestka S, Brewer G (September 2008). "Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decay". Mol. Cell. Biol. 28 (17): 522337.<br /><br />3. Panda AC, Abdelmohsen K, Yoon JH, Martindale JL, Yang X, Curtis J, Mercken EM, Chenette DM, Zhang Y, Schneider RJ, Becker KG, de Cabo R, Gorospe M (Aug 2014). "RNA-binding protein AUF1 promotes myogenesis by regulating MEF2C expression levels". Mol Cell Biol. 34 (16): 310619.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002717-1	M09982	-	100 ug	$370		Add to Cart
BHA21002717-2	M09982-10ug	Sample	10 ug	$99		Add to Cart
BHA21002717-3	M09982-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002717-4	M09982-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002717-5	M09982-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002717-6	M09982-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002717-7	M09982-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002717-8	M09982-FITC	FITC	100 ug	$570		Add to Cart
BHA21002717-9	M09982-HRP	HRP	100 ug	$570		Add to Cart
BHA21002717-10	M09982-APC	APC	100 ug	$670		Add to Cart
BHA21002717-11	M09982-PE	PE	100 ug	$670		Add to Cart
BHA21002717-12	M09982-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002717-13	M09982-carrier-free	carrier-free	100 ug	$370		Add to Cart

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