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Home / Primary Antibodies / Monoclonal Antibodies

Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 3G13)

SKU : BHA21003824

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Mfr.No.	M01272-3, M01272-3-10ug, M01272-3-Biotin, M01272-3-Cy3, M01272-3-Dylight488, M01272-3-Dylight550, M01272-3-Dylight594, M01272-3-FITC, M01272-3-HRP, M01272-3-APC, M01272-3-PE, M01272-3-iFluor647, M01272-3-carrier-free
Alternative Names	ubiquitin specific peptidase 7
Background	This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alteate splicing results in multiple transcript variants.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 3G13) catalog # M01272-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promo
Host	Mouse
Immunogen Region	E.coli-derived human Glutaminase/GLS recombinant protein (Position: K396-N654).
Isotype	Mouse IgG2a
Molecular Weight	56-73 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Ubiquitin carboxyl-terminal hydrolase 7; Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7
Tissue Specificity	Widely expressed. Overexpressed in prostate cancer.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	O94925
Gene Id	2744
Gene Name	GLS
Clone No	Clone: 3G13
Subcellular Location	Nucleus. PML body. Cytoplasm. Chromosome.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB, IHC, ICC, IF, Flow Cytometry
Description	Weste blot, 0.25-0.5 ug/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 ug/ml, Human Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human Flow Cytometry (Fixed), 1-3 ug/1x10⁶ cells, Human

Reference	1. Lynch, D. S., Chelban, V., Vandrovcova, J., Pittman, A., Wood, N. W., Houlden, H. GLS loss of function causes autosomal recessive spastic ataxia and optic atrophy. Ann. Clin. Transl. Neurol. 5: 216-221, 2018. 2. Modi, W. S., Pollock, D. D., Mock, B. A., Banner, C., Renauld, J.-C., Van Snick, J. Regional localization of the human glutaminase (GLS) and interleukin-9 (IL9) genes by in situ hybridization. Cytogenet. Cell Genet. 57: 114-116, 1991. 3. Rumping, L., Tessadori, F., Pouwels, P. J. W., Vringer, E., Wijnen, J. P., Bhogal, A. A., Savelberg, S. M. C., Duran, K. J., Bakkers, M. J. G., Ramos, R. J. J., Schellekens, P. A. W., Kroes, H. Y., and 16 others. GLS hyperactivity causes glutamate excess, infantile cataract and profound developmental delay. Hum. Molec. Genet. 28: 96-104, 2019.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003824-1	M01272-3	-	100 ug	$370		Add to Cart
BHA21003824-2	M01272-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21003824-3	M01272-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003824-4	M01272-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003824-5	M01272-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003824-6	M01272-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003824-7	M01272-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003824-8	M01272-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21003824-9	M01272-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21003824-10	M01272-3-APC	APC	100 ug	$670		Add to Cart
BHA21003824-11	M01272-3-PE	PE	100 ug	$670		Add to Cart
BHA21003824-12	M01272-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003824-13	M01272-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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