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Home / Primary Antibodies / Monoclonal Antibodies

Anti-GFAP Antibody (Monoclonal, G-A-5)

SKU : BHA21006523

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Mfr.No.	MA1045
Alternative Names	Glial fibrillary acidic protein
Background	Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Function	GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Host	Mouse
Immunogen Region	GFAP from pig spinal cord.
Isotype	Mouse IgG1
Molecular Weight	50 kDa
Reactivity	Human, Mouse, Pig, Rat
Reconstitution	Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Glial fibrillary acidic protein;GFAP;Gfap;
Tissue Specificity	Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment. .
Note	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Uniprot	P47819
Gene Id	Human(2670), Mouse(14580), Rat(24387)
Gene Name	GFAP
Clone No	Clone: G-A-5
Sequence	Belongs to the intermediate filament family.
Subcellular Location	Cytoplasm . Associated with intermediate filaments. .

Concentration	Adding 1 ml of PBS buffer will yield a concentration of 100 ug/ml.
Form	Lyophilized
Purification	Ascites
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IF, IHC, IHC-F, WB
Description	Weste blot, 0.5-1ug/ml, Human, mouse, pig, rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.4-1ug/ml, Human, pig, rat, By Heat <br>Immunohistochemistry (Frozen Section), 0.5-1ug/ml, Human, pig, rat, -<br> Immunofluorescence, 2ug/ml, Rat<br>

Reference	1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001. <br /><br />2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Heandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006523-1	MA1045	-	100 ug	$370		Add to Cart

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