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Home / Primary Antibodies / Monoclonal Antibodies

Anti-EEF2 Picoband® Antibody (monoclonal, 5F5)

SKU : BHA21003106

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Mfr.No.	M00830-2, M00830-2-10ug, M00830-2-Biotin, M00830-2-Cy3, M00830-2-Dylight488, M00830-2-Dylight550, M00830-2-Dylight594, M00830-2-FITC, M00830-2-HRP, M00830-2-APC, M00830-2-PE, M00830-2-iFluor647, M00830-2-carrier-free
Alternative Names	dihydrofolate reductase
Background	Eukaryotic elongation factor 2is aproteinthat in humans is encoded by theEEF2gene. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-EEF2 Picoband® Antibody (monoclonal, 5F5) catalog # M00830-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Host	Mouse
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human EEF2/Elongation factor 2, identical to the related mouse and rat sequences.
Isotype	Mouse IgG1
Molecular Weight	95 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Dihydrofolate reductase; DHFR
Tissue Specificity	Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	P13639
Gene Id	1938
Gene Name	EEF2
Clone No	Clone: 5F5
Subcellular Location	Mitochondrion. Cytoplasm

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, ICC, IF, Flow Cytometry
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Huma<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human, Mouse, Rat<br>

Reference	1. Davydova, E., Ho, A. Y. Y., Malecki, J., Moen, A., Enserink, J. M., Jakobsson, M. E., Loenarz, C., Falnes, P. O. Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2). J. Biol. Chem. 289: 30499-30510, 2014. <br /><br />2. Hekman, K. E., Yu, G.-Y., Brown, C. D., Zhu, H., Du, X., Gervin, K., Undlien, D. E., Peterson, A., Stevanin, G., Clark, H. B., Pulst, S. M., Bird, T. D., White, K. P., Gomez, C. M. A conserved eEF2 coding variant in SCA26 leads to loss of translational fidelity and increased susceptibility to proteostatic insult. Hum. Molec. Genet. 21: 5472-5483, 2012.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003106-1	M00830-2	-	100 ug	$370		Add to Cart
BHA21003106-2	M00830-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21003106-3	M00830-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003106-4	M00830-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003106-5	M00830-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003106-6	M00830-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003106-7	M00830-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003106-8	M00830-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21003106-9	M00830-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21003106-10	M00830-2-APC	APC	100 ug	$670		Add to Cart
BHA21003106-11	M00830-2-PE	PE	100 ug	$670		Add to Cart
BHA21003106-12	M00830-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003106-13	M00830-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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