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Home / Primary Antibodies / Monoclonal Antibodies

Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)

SKU : BHA21002169

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Mfr.No.	M00063-2, M00063-2-10ug, M00063-2-Biotin, M00063-2-Cy3, M00063-2-Dylight488, M00063-2-Dylight550, M00063-2-Dylight594, M00063-2-FITC, M00063-2-HRP, M00063-2-APC, M00063-2-PE, M00063-2-iFluor647, M00063-2-carrier-free
Alternative Names	cadherin 1, type 1, E-cadherin (epithelial)
Background	CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southe analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) catalog # M00063-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regul
Host	Mouse
Immunogen Region	E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.
Isotype	Mouse IgG1
Molecular Weight	130 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; CDH1; CDHE; UVO
Tissue Specificity	Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	P12830
Gene Id	999
Gene Name	CDH1
Clone No	Clone: 9G2
Subcellular Location	Trans-Golgi network. Cell membrane. Single-pass type I membrane protein. Endosome. Cell junction.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml<br> Immunocytochemistry/Immunofluorescence, 2ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells<br>

Reference	1.Agiostratidou G., Muros R.M., Shioi J., Marambaud P., Robakis N.K. The cytoplasmic sequence of E-cadherin promotes non-amyloidogenic degradation of A beta precursors. J. Neurochem. 96:1182-1188(2006).<br /><br />2.Frebourg, T., Oliveira, C., Hochain, P., Karam, R., Manouvrier, S., Graziadio, C., Vekemans, M., Hartmann, A., Baert-Desurmont, S., Alexandre, C., Lejeune Dumoulin, S., Marroni, C., and 16 others. Cleft lip/palate and CDH1/E-cadherin mutation in families with hereditary diffuse gastric cancer. J. Med. Genet. 43: 138-142, 2006.<br /><br />3.Mansouri, A., Goodfellow, P. N., Kemler, R. Molecular cloning and chromosomal localization of the human cell adhesion molecule uvomorulin (UVO). (Abstract) Cytogenet. Cell Genet. 46: 655, 1987.<br /><br />4.Mansouri, A., Spurr, N., Goodfellow, P. N., Kemler, R. Characterization and chromosomal localization of the gene encoding the human cell adhesion molecule uvomorulin. Differentiation 38: 67-71, 1988.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002169-1	M00063-2	-	100 ug	$370		Add to Cart
BHA21002169-2	M00063-2-10ug	Sample	10 ug	$99		Add to Cart
BHA21002169-3	M00063-2-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002169-4	M00063-2-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002169-5	M00063-2-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002169-6	M00063-2-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002169-7	M00063-2-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002169-8	M00063-2-FITC	FITC	100 ug	$570		Add to Cart
BHA21002169-9	M00063-2-HRP	HRP	100 ug	$570		Add to Cart
BHA21002169-10	M00063-2-APC	APC	100 ug	$670		Add to Cart
BHA21002169-11	M00063-2-PE	PE	100 ug	$670		Add to Cart
BHA21002169-12	M00063-2-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002169-13	M00063-2-carrier-free	carrier-free	100 ug	$370		Add to Cart

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