Anti-DYKDDDDK (Flag) Affinity Gel

Mfr.No.

20584ES03, 20584ES08, 20584ES25

Description

Anti-DYKDDDDK (Flag) Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with 4% highly cross-linked agarose gel. This product has a high Flag label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Flag labels.

Anti-DYKDDDDK (FLAG) Affinity Gel can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).

Featured

High Specificity—The selection of high quality murine IgG2b monoclonal antibody can specifically adsorb the Flag protein sequence.
high yield and high purity—Obtain high purity Flag label protein
High binding capacity—Capacity ≥1.1mg protein /mL gel
Many types of protein binding—Met-flag-Protein, N-flag fusion protein, C-terminal FLAG Fusion Protein
Widely used—Can be used for protein purification or IP

Isotype

IgG2b

Kit Components

Components No.	Name	20584ES03	20584ES08	20584ES25	20584ES60
20584	Anti-DYKDDDDK (Flag) Affinity Gel	1 mL	5 mL	25 mL	100 mL

Note: In order to facilitate customers to use 1×Flag polypeptide elution, this product package is accompanied by 1×Flag polypeptide (Cat#20572ES).
Based on the different specifications of 1×Flag polypeptide (Cat#20572ES), 1 mL gel package is combined with 5 mg peptide, and other gel specifications are combined with 1 mL: 1 mg ratio of 5 mg specification 1×Flag polypeptide (Cat#20572ES).

Shipping

The products are shipped with ice pack

Note

Protein binding capacity ≥1.1 mg protein/mL Gel

Clone No	1A3

Buffer	TBS，50% glycerin，pH7.4，contains 0.02% (w/v) sodium azide
Storage	Stored at -25~-15℃ for 1 year. Do not freeze in solution without glycerin!

Applications	IP

Product Manual	Manual

Reference	1. Xiao J, Wang S, Chen L, et al. 25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages. Immunity. 2024;57(5):1087-1104.e7. doi:10.1016/j.immuni.2024.03.021(IF:32.4) 2. Zheng Z, Zeng X, Zhu Y, et al. CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alteative splicing and targeting the miR-182-5p/CYP1B1 axis. Mol Cancer. 2024;23(1):4. Published 2024 Jan 6. doi:10.1186/s12943-023-01912-w(IF:37.3)

The SDS-PAGE gel electrophoresis results of Protein purification with Anti-Flag Affinity Gel (20584ES).

Purification of low expression protein by Anti-Flag affinity gel (20584ES) Affinity Gel. a：Flag-N b：Flag-M c：Flag-C M：Protein Marker 1：Flag-C pre-column stock solution 2：Flag-CPost-column penetrating solution 3: elution (pH2.7) 4: elution (pH12 ) 5: elution (1×Flag polypeptide ) 6: purified gel (pH2.7 elution) 7: purified gel (pH12 elution) 8: Purified gel (1×Flag polypeptide elution)

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Combination SKU	Supplier No.	Size	Your price
BHA20800003-1	20584ES03	1mL	$385	Add to Cart
BHA20800003-2	20584ES08	5mL	$960	Add to Cart
BHA20800003-3	20584ES25	25mL	$2410	Add to Cart

Anti-DYKDDDDK (Flag) Affinity Gel

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