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Home / Primary Antibodies / Monoclonal Antibodies

Anti-DDX1 Picoband® Antibody (monoclonal, 3I10)

SKU : BHA21003157

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Mfr.No.	M03727, M03727-10ug, M03727-Biotin, M03727-Cy3, M03727-Dylight488, M03727-Dylight550, M03727-Dylight594, M03727-FITC, M03727-HRP, M03727-APC, M03727-PE, M03727-iFluor647, M03727-carrier-free
Alternative Names	eukaryotic translation initiation factor 6
Background	ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. It is mapped to 2p24.3. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution pattes, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-DDX1 Picoband® Antibody (monoclonal, 3I10) catalog # M03727. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved
Host	Mouse
Immunogen Region	E.coli-derived human DDX1 recombinant protein (Position: K562-F740).
Isotype	Mouse IgG1
Molecular Weight	86 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Eukaryotic translation initiation factor 6; eIF-6; B (2)GCN homolog; B4 integrin interactor; CAB; p27 (BBP); EIF6; EIF3A; ITGB4BP; OK/SW-cl.27
Tissue Specificity	Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	Q92499
Gene Id	1653
Gene Name	DDX1
Clone No	Clone: 3I10
Subcellular Location	nucleolus. Cytoplasm

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.25ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, uman, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human<br> Immunofluorescence, 5 ug/ml, Mouse<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ (October 2002). "An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K". The Joual of Biological Chemistry. 277 (43): 404039. <br /><br />2. Cho WJ, Yoon WJ, Moon CH, Cha SJ, Song H, Cho HR, Jang SJ, Chung DK, Jeong CS, Park JW (November 2002). "Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5". The Joual of Biological Chemistry. 277 (44): 4148996. <br /><br />3. Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Research. 15 (1112): 92334.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003157-1	M03727	-	100 ug	$370		Add to Cart
BHA21003157-2	M03727-10ug	Sample	10 ug	$99		Add to Cart
BHA21003157-3	M03727-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003157-4	M03727-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003157-5	M03727-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003157-6	M03727-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003157-7	M03727-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003157-8	M03727-FITC	FITC	100 ug	$570		Add to Cart
BHA21003157-9	M03727-HRP	HRP	100 ug	$570		Add to Cart
BHA21003157-10	M03727-APC	APC	100 ug	$670		Add to Cart
BHA21003157-11	M03727-PE	PE	100 ug	$670		Add to Cart
BHA21003157-12	M03727-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003157-13	M03727-carrier-free	carrier-free	100 ug	$370		Add to Cart

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