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Home / Primary Antibodies / Monoclonal Antibodies

Anti-COPE Antibody Picoband® (monoclonal, 9B6)

SKU : BHA21002277

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Mfr.No.	M04544, M04544-10ug, M04544-Biotin, M04544-Cy3, M04544-Dylight488, M04544-Dylight550, M04544-Dylight594, M04544-FITC, M04544-HRP, M04544-APC, M04544-PE, M04544-iFluor647, M04544-carrier-free
Alternative Names	coatomer protein complex subunit epsilon
Background	Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alteatively spliced transcript variants encoding different isoforms have been identified.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-COPE Antibody Picoband® (monoclonal, 9B6) catalog # M04544. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The
Host	Mouse
Immunogen Region	E. coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.
Isotype	Mouse IgG2a
Molecular Weight	34 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Coatomer subunit epsilon; Epsilon-coat protein; Epsilon-COP; COPE
Tissue Specificity	Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

Uniprot	O14579
Gene Id	11316
Gene Name	COPE
Clone No	Clone: 9B6
Subcellular Location	Golgi apparatus membrane. Peripheral membrane protein. Cytoplasmic side. Cytoplasm. COPI-coated vesicle membrane.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml<br> Immunocytochemistry/Immunofluorescence, 5ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells<br>

Reference	1. "Entrez Gene: COPE coatomer protein complex, subunit epsilon".<br /><br />2. Shima DT, Scales SJ, Kreis TE, Pepperkok R (November 1999). "Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes". Curr. Biol. 9 (15): 8214.<br /><br />3. Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Ko B, Birchmeier W, Lehrach H, Wanker EE (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 95768.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002277-1	M04544	-	100 ug	$370		Add to Cart
BHA21002277-2	M04544-10ug	Sample	10 ug	$99		Add to Cart
BHA21002277-3	M04544-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002277-4	M04544-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002277-5	M04544-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002277-6	M04544-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002277-7	M04544-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002277-8	M04544-FITC	FITC	100 ug	$570		Add to Cart
BHA21002277-9	M04544-HRP	HRP	100 ug	$570		Add to Cart
BHA21002277-10	M04544-APC	APC	100 ug	$670		Add to Cart
BHA21002277-11	M04544-PE	PE	100 ug	$670		Add to Cart
BHA21002277-12	M04544-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002277-13	M04544-carrier-free	carrier-free	100 ug	$370		Add to Cart

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