Recombinant human antibody recognizing the CD3 binding region on immune cells.
assay conditions: IL-2 luciferase Jurkat reporter cells were incubated with anti-CD3 (BPS Cat. No 71274) in SF-RPMI for 24 hours. BPS luciferase reagent was added and luminescence was quantified. RLU = Relative Luminescence Unit normalized to wells with RPMI only.
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Isotype
IgG1
Molecular Weight
Heavy Chain: 52 kDa, Light Chain: 26 kDa
Species
Human
Tag
none
Note
Avoid freeze/thaw cycles.
Format
Aqueous buffer solution
Purification
Protein A affinity chromatography from HEK293 supernatants.
Shipping Temperature
-80°C (dry ice)
Storage Stability
Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.