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Home / Primary Antibodies / Monoclonal Antibodies

Anti-CD19 IgG Antibody

SKU : BHA18200057

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BPS Bioscience

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Mfr.No.	100981-2
Clonality	Monoclonal
Description	This purified recombinant monoclonal antibody is a human Anti-CD19 IgG which recognizes CD19 antigen. The variable regions for this antibody are based on an FDA-approved drug. This antibody has been tested for specific binding affinity to purified human CD19 protein (BPS Bioscience, #101015) in a colorimetric ELISA binding assay. assay conditions: Purified human his-tagged CD19 was bound to clear 96-well nickel plate overnight in 4°C (1 ug/mL). Upon washing and blocking wells for 1 hour at room temp, serial dilutions of anti-CD19 (200 nM to 0nM in 3-fold dilutions) were incubated in each well for 1 hr at room temp (slow shaking). Next, wells were washed and incubated with anti-human IgG-HRP for 1 hour at room temp (slow shaking) then washed again. For detection, the wells were incubated with Colorimetric HRP Substrate (BPS Bioscience #79651) for 1-10 minutes until a blue color developed in the positive control. The reaction was then quenched with equal volume of 1N HCL and absorbance read at 450 nm.
Formulation	8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Molecular Weight	HC: ~49.9 kDa; LC: ~23.9 kDa
Species	Human
Synonyms	B4 antibody, CVID3 antibody, 100981-1, 100981-2
Note	Avoid freeze/thaw cycles.

Uniprot	P15391

Format	Aqueous buffer solution
Purification	Protein A affinity purification from HEK293 cells.
Purity	>=90%
Shipping Temperature	-80°C (dry ice)
Storage Stability	At least 12 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA18200057	100981-2	100 ug	$506		Add to Cart

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