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Home / Primary Antibodies / Monoclonal Antibodies

Anti-APPL/APPL1 Antibody Picoband® (monoclonal, 5G11)

SKU : BHA21003107

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Mfr.No.	M02381, M02381-10ug, M02381-Biotin, M02381-Cy3, M02381-Dylight488, M02381-Dylight550, M02381-Dylight594, M02381-FITC, M02381-HRP, M02381-APC, M02381-PE, M02381-iFluor647, M02381-carrier-free
Alternative Names	ATP citrate lyase
Background	DCC-interacting protein 13-alpha (APPL1) is a protein that in humans is encoded by the APPL1 gene. The APPL1 gene is mapped to 3q21.1-p13.3. It is said to contain 709 amino acids and share 54% amino acid identity with APPL2. APPL is highly expressed in skeletal muscle, heart, ovary, and pancreas, tissues in which AKT2 mRNA is abundant. It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-APPL/APPL1 Antibody Picoband® (monoclonal, 5G11) catalog # M02381. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Host	Mouse
Immunogen Region	E.coli-derived human APPL/APPL1 recombinant protein (Position: K91-R668).
Isotype	Mouse IgG2a
Molecular Weight	85 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme; ACLY
Tissue Specificity	Widely expressed. Highest level in placenta, liver, muscle and kidney.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Uniprot	Q9UKG1
Gene Id	26060
Gene Name	APPL1
Clone No	Clone: 5G11
Subcellular Location	Cytoplasm.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, IHC, Flow Cytometry
Description	Weste blot, 0.25-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br>

Reference	1. Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR (Oct 1999). "Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2". Oncogene 18 (35): 48918. doi:10.1038/sj.onc.1203080. PMID 10490823. <br /><br />2. Nechamen CA, Thomas RM, Dias JA (Nov 2006). "APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex". Mol Cell Endocrinol 260-262: 939. doi:10.1016/j.mce.2006.08.014.PMC 1782224. PMID 17030088. <br /><br />3. Entrez Gene: APPL1 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1".

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003107-1	M02381	-	100 ug	$370		Add to Cart
BHA21003107-2	M02381-10ug	Sample	10 ug	$99		Add to Cart
BHA21003107-3	M02381-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003107-4	M02381-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003107-5	M02381-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003107-6	M02381-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003107-7	M02381-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003107-8	M02381-FITC	FITC	100 ug	$570		Add to Cart
BHA21003107-9	M02381-HRP	HRP	100 ug	$570		Add to Cart
BHA21003107-10	M02381-APC	APC	100 ug	$670		Add to Cart
BHA21003107-11	M02381-PE	PE	100 ug	$670		Add to Cart
BHA21003107-12	M02381-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003107-13	M02381-carrier-free	carrier-free	100 ug	$370		Add to Cart

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