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Home / Primary Antibodies / Monoclonal Antibodies

Anti-APOBEC3G Antibody Picoband® (monoclonal, 6C2)

SKU : BHA21002162

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Mfr.No.	M00708, M00708-10ug, M00708-Biotin, M00708-Cy3, M00708-Dylight488, M00708-Dylight550, M00708-Dylight594, M00708-FITC, M00708-HRP, M00708-APC, M00708-PE, M00708-iFluor647, M00708-carrier-free
Alternative Names	apolipoprotein B mRNA editing enzyme catalytic subunit 3G
Background	APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) catalog # M00708. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against vif-deficient HIV-1. After the penetration
Host	Mouse
Immunogen Region	E.coli-derived human APOBEC3G recombinant protein (Position: E191-N384).
Isotype	Mouse IgG1
Molecular Weight	46 kDa
Reactivity	Human
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	DNA dC->dU-editing enzyme APOBEC-3G; APOBEC-related cytidine deaminase; APOBEC-related protein; ARCD; APOBEC-related protein 9; ARP-9; CEM-15; CEM15; Deoxycytidine deaminase; A3G; APOBEC3G; MDS019
Tissue Specificity	Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. Exists only in the LMM form in peripheral blood-derived resting CD4 T-cells and monocytes, both of which are refractory to HIV-1 infection. LMM is converted to a HMM complex when resting CD4 T-cells are activated or when monocytes are induced to differentiate into macrophages. This change correlates with increased susceptibility of these cells to HIV-1 infection.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Uniprot	Q9HC16
Gene Id	60489
Gene Name	APOBEC3G
Clone No	Clone: 6C2
Subcellular Location	Nucleus. Cytoplasm. P-body.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.5ug/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml<br> Immunocytochemistry/Immunofluorescence, 2ug/ml<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells<br>

Reference	1.Chelico L, Prochnow C, Erie DA, Chen XS, Goodman MF (2011)."Structural model for deoxycytidine deaminaton mechanism of the HIV-1 inactivation enzyme APOBEC3G". The Joual of Biological Chemistry 285(21): 16195205.<br /><br />2.Li X, Ma J, Zhang Q, Yin X, Zhai C, You X, Yu L, Guo F, Zhao L, Li Z, Zeng Y, Cen S (2011). "Functional analysis of the two cytidine deaminase domains in APOBEC3G". Virology 414 (2): 130136.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002162-1	M00708	-	100 ug	$370		Add to Cart
BHA21002162-2	M00708-10ug	Sample	10 ug	$99		Add to Cart
BHA21002162-3	M00708-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002162-4	M00708-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002162-5	M00708-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002162-6	M00708-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002162-7	M00708-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002162-8	M00708-FITC	FITC	100 ug	$570		Add to Cart
BHA21002162-9	M00708-HRP	HRP	100 ug	$570		Add to Cart
BHA21002162-10	M00708-APC	APC	100 ug	$670		Add to Cart
BHA21002162-11	M00708-PE	PE	100 ug	$670		Add to Cart
BHA21002162-12	M00708-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002162-13	M00708-carrier-free	carrier-free	100 ug	$370		Add to Cart

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