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Home / Primary Antibodies / Monoclonal Antibodies

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)

SKU : BHA21006567

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Mfr.No.	MA1105
Alternative Names	adaptor-related protein complex 2, alpha 1 subunit
Background	Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Clonality	Monoclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Function	AP2A1: Component of the adaptor protein complex 2 (AP-2).Adaptor protein complexes function in protein transport viatransport vesicles in different membrane traffic pathways. Adaptorprotein complexes are vesicle coat components and appear to beinvolved in
Host	Mouse
Immunogen Region	AP-2 adaptor polypeptides from bovine brain.
Isotype	Mouse IgG2a
Molecular Weight	108 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Adaptor protein complex AP-2, alpha 1 subunit (Predicted) ;Protein Ap2a1 ;Ap2a1 ;Ap2a1_predicted ;rCG_54084 ;
Tissue Specificity	AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells.
Note	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Uniprot	D3ZUY8
Gene Id	Human(160), Mouse(11771), Rat(308578)
Gene Name	AP2A1
Clone No	Clone: 100/2
Sequence	Belongs to the adaptor complexes large subunit family.
Subcellular Location	AP2A1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from intealizing CCVs and to disengage from sitesof endocytosis seconds before intealization of the nascent CCV.\|Ap2a1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from intealizing CCVs and to disengage from sitesof endocytosis seconds before intealization of the nascent CCV.

Concentration	Adding 1 ml of PBS buffer will yield a concentration of 100 ug/ml.
Form	Lyophilized
Purification	Ascites
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB
Description	Weste blot, 0.5-1ug/ml, Human, mouse, rat<br>

Reference	1. Robinson, M. S. : Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins). J. Cell Biol. 108: 833-42, 1989. <br /><br />2. Waelter, S.; Scherzinger, E.; Hasenbank, R.; Nordhoff, E.; Lurz, R.; Goehler, H.; Gauss, C.; Sathasivam, K.; Bates, G. P.; Lehrach, H.; Wanker, E. E. : The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis. Hum. Molec. Genet. 10: 1807-1817, 2001.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006567-1	MA1105	-	100 ug	$370		Add to Cart

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