Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa

Mfr.No.	SMC-172, SMC-172D, SMC-172C, SMC-172D-A390, SMC-172D-A488, SMC-172D-A594, SMC-172D-ALP, SMC-172D-APC, SMC-172D-BI, SMC-172D-FITC, SMC-172D-HRP, SMC-172D-PCP, SMC-172D-RPE, SMC-172S
Immunogen Species	Mouse
Alternative Names	Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody
Categories	Antibodies
Cellular Localization	Endoplasmic Reticulum, Cytoplasm
Certificate Of Analysis	1 ug/ml of SMC-172 was sufficient for detection of Aha1 in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rat
Gross Weight (kg)	1.4
Immunogen	Mouse Aha1, Mus Musculus (Mouse)
Immunogen Region	Recombinant Full Length Mouse Aha1 Protein
Isotype	IgG2a Kappa
Launch Date	2010/01/01
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Mus Musculus (Mouse)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	Q8BK64
Accession Number	NP_666148.1
Gene Id	217737
Target	AHA1
Clone No	25F2.D9
Research Area	Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking

Buffer	0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
Concentration	1.2 mg/ml
Dilutions	WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~38kDa. Can run up to 45kDa on SDS Page.
Storage	-20ºC
Storage Buffer	0.02M potassium phosphate, 0.15M sodium chloride, pH7.2 *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC, IP, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution	WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11901096-1	SMC-172D	Unconjugated	100 ug	$345	Add to Cart
BHA11901096-2	SMC-172C	Unconjugated	25 ug	$156	Add to Cart
BHA11901096-3	SMC-172D-A390	ATTO 390	100 ug	$399	Add to Cart
BHA11901096-4	SMC-172D-A488	ATTO 488	100 ug	$398	Add to Cart
BHA11901096-5	SMC-172D-A594	ATTO 594	100 ug	$398	Add to Cart
BHA11901096-6	SMC-172D-ALP	Alkaline Phosphatase	100 ug	$392	Add to Cart
BHA11901096-7	SMC-172D-APC	APC	100 ug	$397	Add to Cart
BHA11901096-8	SMC-172D-BI	Biotin	100 ug	$394	Add to Cart
BHA11901096-9	SMC-172D-FITC	FITC	100 ug	$389	Add to Cart
BHA11901096-10	SMC-172D-HRP	HRP	100 ug	$385	Add to Cart
BHA11901096-11	SMC-172D-PCP	PerCP	100 ug	$397	Add to Cart
BHA11901096-12	SMC-172D-RPE	RPE	100 ug	$395	Add to Cart
BHA11901096-13	SMC-172S	Unconjugated	12 ug	$44	Add to Cart

AHA1 Antibody, Clone 25F2.D9 - SMC-172