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Home / Proteins & Peptide

MIP-5/CCL15, Human

SKU : BHP18500091

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Mfr.No.	IX02838, IX02838-1, IX02838-25
Description	CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1Î³, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20°C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Molecular Weight	Approximately 10.2 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	Human
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Endotoxin Level	Less than 1 EU/ug of rHuMIP-5/CCL15 as determined by LAL method.
Bioactivity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.

Synonyms	MIP-5/CCL15, Human;
Sequence	QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQSIPCSLMKSYF ETSSECSKPG?VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPYSI

Purity	97 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500091-1	IX02838-1	1mg	$2621		Add to Cart
BHP18500091-2	IX02838-25	25ug	$199		Add to Cart

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