TNP coated surface on the TNP Coated 96 Well Breakable Strip Plates can be used as biomarkers of immunotoxicity, assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing and determination of immune status relative to controls.
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| Mfr.No. | MG30F-DNP |
| Description | The DNP Coated Plates are offered with 12 breakable strips of 8 wells assembled on single well holding frame. Clear breakable strips are manufactured in pure polystyrene with low fluorescence and can be used for ELISA assays. The DNP Coated 96 Well Plates Breakable Strips are designed to be easily breakable by the user and repositioned in the frame; the radius edged inner bottom of the wells improves the efficiency of washing. The DNP Coated 96 Well Breakable Strip Plates comply with SBS standards and the design assures a good performance in automatic processing plant.
White and black DNP Coated 96 Well Breakable Strips Plates can be used for Luminescence and Fluorescence assays and are AVAILABLE BY ORDER ONLY. Reagents supplied upon request. Biomat other 96 Well Plates for Immunotoxicity ELISA Tests:
FOR RESEARCH USE ONLY |
| Features | • used as biomarkers of immunotoxicity • assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing • determination of immune status relative to controls |
| Plate Type | Breakable Plates |
| Specification | Available configurations 96-well microplates with 12 removable 8-well strips. Coating DNP-BSA is coated using 100 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability. Binding capacity Microplate was saturated with mouse IgG anti DNP at a concentration of 0.125 µg/ml (12.5 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate (see Technical notes 40 and Figure 1 and 2 for data and experiment details). The Biomat DNP microplate shows a nominal binding capacity of ~ 0.125 µg/ml of mouse IgG anti DNP Uniformity Microplates show a CV% less than 5 when used as a sandwich of mouse IgG anti DNP in an ELISA format using goat anti mouse IgG -HRP as detector and TMB as substrate. Storage and Stability The microplates, under the indicated storage conditions 2-8 °C, are stable until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date. |
| Surface | DNP Coated |
| Technical Notes | 1. Add 100 µl of different concentrations of monoclonal mouse IgG anti DNP (Merck-Sigma cat. # Mab 2223 at 1 mg/ml), from 0.0005 to 0.500 µg/ml, diluted in Sample Diluent, (Biomat code 400-1-100) to the wells of DNP coated plate and incubate for 60 minutes at room temperature 2. Empty the wells and wash with Wash Buffer, (Biomat code 200-1-100) four times 3. Add 100 µl /well of goat anti-mouse IgG -HRP (Jackson ImmunoResearch code 115-035-003), diluted 1:25,000 in Diluent for HRP conjugate, (Biomat code 400-2-100) and incubate for 60 minutes at room temperature 4. Empty the wells and wash with Wash Buffer, (Biomat code 200-1-100) four times 5. Add 100 µl /well of TMB substrate solution, (Biomat code 500-1-100) and incubate 15 minutes at room temperature 6. Stop the substrate reaction by adding 100 µl /well of sulphuric acid, (Biomat code 600-1-100) and read the optical density values at 450 nm The data show that a plateau has got starting with a mouse IgG anti DNP concentration of 0.125 μg/ml. This concentration means the well binding capacity we can express as: - μg/well = 0.0125 (12.5 ng/well)  Figure 2: the figure gives an idea of the dilution factor to apply to the serum/plasma of the immunized mouse under evaluation; where k means a dilution of 1:1,000 
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