The DMEM:Ham’s F12 is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham’s F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less. This special medium has been adapted to support the proliferation of NCI-H295R cells. The final concentration of bovine serum is below 1%.
Maintenance
Immediately after delivery, store the medium at 4°C to 8° C in the dark. Do not heat the medium over 37°C or use uncontrollable sources of heat (e.g. microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and warm it up. Do not freeze the medium. This can result in high salt concentrations by freezing out pure water which will cause irreversible damage.
Short Description
DMEM:Hams F12 (1:1) ready-to-use, for NCI-H295R supplemented with L-glutamine, 15 mM Hepes, 0.00625 mg/ml insulin, 0.00625 mg/ml transferrin, 6.25 ng/ml selenium, 1.25 mg/ml bovine serum albumin, 0.00535 mg/ml linoleic acid and 2.5% Nu-Serum I; Shelf life 8 weeks from date of manufacture