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Home / Proteins & Peptide

MCP-3/CCL7, Human

SKU : BHP18500087

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Mfr.No.	IX02833, IX02833-1, IX02833-10
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Description	MCP2 and CCL7 are two monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and CCL7 are members of the CC family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. CCL7 also shares 58% amino acid identity with MCP2.Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Molecular Weight	Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	Human
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Endotoxin Level	Less than 1 EU/ug of rHuMCP-3/CCL7 as determined by LAL method.
Bioactivity	Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Synonyms	MCP-3/CCL7, Human;
Sequence	QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREAVIFKTKLDKE ICADPTQKWV?QDFMKHLDKK TQTPKL

Purity	97 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500087-1	IX02833-1	1mg	$2621		Add to Cart
BHP18500087-2	IX02833-10	10ug	$199		Add to Cart

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