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Home / VECTOR &VIRUS / Lentivirus

PRE Reporter Lentivirus

SKU : BHV19400069

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Mfr.No.	LTV-0074, LTV-0074-1S, LTV-0074-2S, LTV-0074-3S, LTV-0074-4S, LTV-0074-5S, LTV-0074-6S
Description	PRE Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse progesterone signaling pathway in mammalian cells. The progesterone response element (PRE) reporter lentivirus is constructed with tandem repeats of the consensus progesterone receptor DNA-binding element which is transcriptionally responsive to progesterone signaling pathway activity. The physiological effects of progesterone are primarily mediated through progesterone receptor (PR) activation followed by receptor binding to a specific PRE, resulting in the transcription of target genes. Compared to PR-A, isoform PR-B is a transcriptional activator. The PR-B activation is stimulated by activation of MAPK-ERK pathway in the presence of its ligand progesterone. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying progesterone signaling pathways in difficult-to-transfect cells including primary and/or thawed cells.
Promoter	EF1a
Serotype	VSV-G
Reporter	Firefly Luc, RFP, GFP
Response Element	Progesterone response elements (PRE)
Selection	Blasticidin, Puromycin
Signaling pathway	Progesterone Receptor (PR) Signaling Pathway
Species	Human/mouse
Applications Range	Monitor transcriptional activity of progesterone receptor signaling pathway in mammalian cells, screen for activators or inhibitors of progesterone signaling pathway. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc. The progesterone response element (PRE) reporter lentivirus is constructed with tandem repeats of the consensus progesterone receptor DNA-binding element which is transcriptionally responsive to progesterone signaling pathway activity in mammalian cells. The physiological effects of progesterone are primarily mediated through progesterone receptor (PR) activation followed by receptor binding to a specific PRE, resulting in the transcription of target genes. Compared to PR-A, isoform PR-B is a transcriptional activator. The PR-B activation is stimulated by activation of MAPK-ERK pathway in the presence of its ligand progesterone.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Promoter	Your price	Quantity
BHV19400069-1	LTV-0074-1S	5x10^6	Puromycin	GFP	EF1a	$595		Add to Cart
BHV19400069-2	LTV-0074-2S	5x10^6	Blasticidin	RFP	EF1a	$595		Add to Cart
BHV19400069-3	LTV-0074-3S	2x10^6	Puromycin	Firefly Luc	EF1a	$595		Add to Cart
BHV19400069-4	LTV-0074-4S	2x10^6	Blasticidin	Firefly Luc	EF1a	$595		Add to Cart
BHV19400069-5	LTV-0074-5S	5x10^6	Blasticidin	GFP	EF1a	$595		Add to Cart
BHV19400069-6	LTV-0074-6S	5x10^6	Puromycin	RFP	EF1a	$595		Add to Cart

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