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Home / VECTOR &VIRUS / Lentivirus

GITR/NFκB Reporter Lentivirus

SKU : BHV19400184

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Mfr.No.	TRV-0001, TRV-0001-1S, TRV-0001-2S, TRV-0001-3S, TRV-0001-4S, TRV-0001-5S, TRV-0001-6S, TRV-0001-7S, TRV-0001-8S
Description	The GITR/NFκB Immunotherapy Reporter is designed to measure the transcriptional activation of the NFκB pathway in response to modulation of GITR expressed on the cell surface. The all-in-one reporter construct allows the user to easily generate stable reporter cell lines which constitutively express a cell surface receptor/ligand of interest (GITR) and drug selection marker (Puro or BSD), and conditionally express a fluorescent (GFP or RFP) and secreted Gaussia luciferase (GLuc) dual reporter in response to transcriptional activation of the specific signaling pathway (NFκB). Constructs with constitutively expressed fluorescent proteins instead of a drug selection marker are also available for users who prefer to isolate transduced cells by FACS. This can be desirable, for example, in cases where the cells are difficult to culture or need to be rapidly transplanted in vivo. The GITR/NFκB Reporter provides a useful means for the user to assay T or NK cell activity related to GITR signaling in the cells of their choosing. This product is supplied as high-titer lentiviral particles, which have been purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped and are ideal for difficult-to-transfect cells including primary or thawed cells. Product Format: High-titer Lentiviral Particles, third generation, VSV-G pseudotyped Bulk solution: Serum-free RPMI-1640, frozen solution Unit of measure: One vial Volume: 380 μl/vial Viral Particles (VP) Concentration: 1×10^9 VP/ml cDNA Expressed: Human GITR (C-terminal Flag) Response Elements: NFκB response elements
Promoter	EF1α
Titer	1-2×10^6 TU/vial, depending on the variant
Reporter	RFP, RFP-P2A-GLuc, GFP-P2A-GLuc, GFP
Selection	RFP, GFP, Blasticidin, Puromycin
Applications Range	T cell Activation. The product can be used to generate stable cell lines that read out GITR signaling through downstream activity of NFκB, for example in stable T or NK cell lines. The fluorescent and luminescent dual reporter provides flexibility in the readout approach. GLuc is secreted, allowing the user to detect it in various biological media. Generally, the product is useful for analyzing T cell activity as a function of GITR stimulation/inhibition.
Virus Type	Immunotherapy Reporter Lentivirus
Storage	-80oC

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Combination SKU	Supplier No.	Product Name	Reporter	Selection	Amount (TU)	Your price	Quantity
BHV19400184-1	TRV-0001-1S	GITR-GFP/NFκB-RFP	RFP	GFP (constitutively expressed)	2x10^6	$895		Add to Cart
BHV19400184-2	TRV-0001-2S	GITR-RFP/NFκB-GFP	GFP	RFP (constitutively expressed)	2x10^6	$895		Add to Cart
BHV19400184-3	TRV-0001-3S	GITR-GFP/NFκB-GLuc	GLuc	GFP (constitutively expressed)	2x10^6	$895		Add to Cart
BHV19400184-4	TRV-0001-4S	GITR-RFP/NFκB-GLuc	GLuc	RFP (constitutively expressed)	2x10^6	$895		Add to Cart
BHV19400184-5	TRV-0001-5S	GITR-Puro/NFκB-GFP-GLuc	GFP-P2A-GLuc	Puromycin	1x10^6	$895		Add to Cart
BHV19400184-6	TRV-0001-6S	GITR-BSD/NFκB-GFP-GLuc	GFP-P2A-GLuc	Blasticidin	1x10^6	$895		Add to Cart
BHV19400184-7	TRV-0001-7S	GITR-Puro/NFκB-RFP-GLuc	RFP-P2A-GLuc	Puromycin	1x10^6	$895		Add to Cart
BHV19400184-8	TRV-0001-8S	GITR-BSD/NFκB-RFP-GLuc	RFP-P2A-GLuc	Blasticidin	1x10^6	$895		Add to Cart

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