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Home / VECTOR &VIRUS / Lentivirus

GECI Reporter Lentivirus

SKU : BHV19400050

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Mfr.No.	LTV-0054, LTV-0054-1S, LTV-0054-2S, LTV-0054-3S, LTV-0054-4S
Description	Genetically encoded Ca2+ indicator (GECI) can be used for recording the cytosolic Ca2+ in Ca2+ sensitive cells of living mammals. The neuron-specific promoter human synuclein (Syn) driven JGCaMP7b or Red-shift JYCaMP1s calcium sensor can be used for recording calcium signaling of neuronal cells in vitro. CMV promoter-driven GECIs enable measurement of cytosolic Ca2+ in cardiac myocyte and kidney cells in real time. A potential application for the purified GECI lentivirus could include infecting brain cortex neurons to interrogate the neuron activity in vivo. The drug selection marker (puromycin or blasticidin) provides a convenient tool to establish stable cell lines. The product is supplied as a high quality pre-packaged lentivirus, which can be used to establish stable cells within ~10 days in order to visualize Ca2+ signaling with fluorescence. The lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for use with difficult-to-transfect cells including primary and/or thawed cells.
Promoter	CMV, Syn
Serotype	VSV-G
Reporter	JGCamP, JYCamP
Selection	Puromycin
Signaling pathway	Calcium signaling
Species	Human/mouse
Applications Range	Genetically encoded Ca2+ indicator (GECI) can be used for recording the cytosolic Ca2+ in Ca2+-sensitive cells of living mammals. The neuron-specific promoter human synuclein (Syn) driven JGCaMP7b or Red-shift JYCaMP1s calcium sensor can be used for recording calcium signaling of neuronal cells in vitro. CMV promoter-driven GECIs enable measurement of cytosolic Ca2+ in cardiac myocyte and kidney cells in real time. Potential other applications could include infecting brain cortex neurons to interrogate the neuron activity in vivo. The drug selection marker (puromycin or blasticidin) provides a convenient tool to establish stable cell lines.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Promoter	Your price	Quantity
BHV19400050-1	LTV-0054-1S	2x10^6	Puromycin	JGCamP	CMV	$595		Add to Cart
BHV19400050-2	LTV-0054-2S	2x10^6	Puromycin	JYCamP	CMV	$595		Add to Cart
BHV19400050-3	LTV-0054-3S	2x10^6	Puromycin	JGCamP	Syn	$595		Add to Cart
BHV19400050-4	LTV-0054-4S	2x10^6	Puromycin	JYCamP	Syn	$595		Add to Cart

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