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Home / VECTOR &VIRUS / Lentivirus

Cell Cycle Reporter Lentivirus

SKU : BHV19400048

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Mfr.No.	LTV-0052, LTV-0052-1S, LTV-0052-2S
Description	Cell Cycle Reporter Lentivirus: Fluorescent ubiquitination-based cell cycle indicator (FUCCI) is a set of fluorescent probes which enables visualization of cell cycle progression in living cells without fixation. Human CDT1 (30-120), which accumulates only in the G1 phase of the cell cycle, is fused at the C-terminus of RFP, and human Geminin (Gemn 1-110), which accumulates in G2/M phases, is fused at the C-terminus of GFP. In our lentiviral vector, the CMV promoter drives expression of RFP-CDT1, GFP-Gemn, and the drug selection marker (puromycin or blasticidin) as a single mRNA transcript. Self-cleaving T2A and P2A sequences separate the RFP-CDT1GFP-Gemn and GFP-GemnSelection marker, respectively, resulting in three separate proteins. This arrangement allows for even expression of RFP-CDT1 and GFP-Gemn enabling one to precisely monitor all phases of the cell cycle using fluorescence in either stable cell lines or cells that have been transiently transduced with the lentivirus. The product is supplied as high quality pre-packaged lentivirus which is purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped and ideal for use in a wide range of mammalian cells, including difficult-to-transfect cells such as primary or thawed cells, and are capable of supporting studies in stem cell biology and human pluripotent stem cells.
Promoter	CMV
Serotype	VSV-G
Reporter	hCDT1-RFP + hGemn-GFP
Selection	Blasticidin, Puromycin
Signaling pathway	Cell cycle
Species	Human/mouse
Applications Range	A simple, fluorescence-based method to visualize cell cycle progression. The pre-packaged lentivirus is a convenient format that allows you to create stable cell lines easily within about 10 days after transducing your cells. Simply add the Fucci lentivirus to the cells and wait 2-3 days, then select with puromycin or blasticidin (depending on the product) for 2-3 days. After this, the cells can be visualized under fluorescence microscope and can be seeded to perform cell cycle assays based on your application.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Promoter	Your price	Quantity
BHV19400048-1	LTV-0052-1S	2x10^6	Puromycin	hCDT1-RFP + hGemn-GFP	CMV	$595		Add to Cart
BHV19400048-2	LTV-0052-2S	2x10^6	Blasticidin	hCDT1-RFP + hGemn-GFP	CMV	$595		Add to Cart

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