CD16A/NFAT Reporter Lentivirus

Mfr.No.	TRV-0003, TRV-0003-1S, TRV-0003-2S, TRV-0003-3S, TRV-0003-4S, TRV-0003-5S, TRV-0003-6S, TRV-0003-7S, TRV-0003-8S
Description	The CD16A/NFAT Immunotherapy Reporter is designed to measure the transcriptional activation of the NFAT pathway in response to modulation of CD16A expressed on the cell surface. The all-in-one reporter construct allows the user to easily generate stable reporter cell lines which constitutively express a cell surface receptor/ligand of interest (CD16A) and drug selection marker (Puro or BSD), and conditionally express a fluorescent (GFP or RFP) and secreted Gaussia luciferase (GLuc) dual reporter in response to transcriptional activation of the specific signaling pathway (NFAT). Constructs with constitutively expressed fluorescent proteins instead of a drug selection marker are also available for users who prefer to isolate transduced cells by FACS. This can be desirable, for example, in cases where the cells are difficult to culture or need to be rapidly transplanted in vivo. The CD16A/NFAT Reporter provides a useful means for the user to assay cell activity related to CD16A signaling in the cells of their choosing. This product is supplied as high-titer lentiviral particles, which have been purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped and are ideal for difficult-to-transfect cells including primary or thawed cells. Product Format: High-titer Lentiviral Particles, third generation, VSV-G pseudotyped Bulk solution: Serum-free RPMI-1640, frozen solution Reporter Expression System: Tandem repeats of response elements coupled to minimal TATA promoter and upstream proprietary enhancer
Promoter	EF1α
Titer	1-2×10^6 TU/vial, depending on the variant
Reporter	RFP, RFP-P2A-GLuc, GFP-P2A-GLuc, GFP
Selection	Blasticidin, Puromycin
Applications Range	Antibody dependent cell-mediated cytotoxicity (ADCC) assay. The product can be used to generate stable cell lines that read out CD16A signaling through downstream activity of NFAT. The fluorescent and luminescent dual reporter provides flexibility in the readout approach. GLuc is secreted, allowing the user to detect it in various biological media. Generally, the product is useful for analyzing cell activity as a function of CD16A stimulation/inhibition.
Virus Type	Immunotherapy Reporter Lentivirus
Storage	-80oC

Product Manual	Datasheet

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Product Name	Your price
BHV19400182-1	TRV-0003-1S	2x10^6	GFP (constitutively expressed)	RFP	CD16A-GFP/NFAT-RFP	$895	Add to Cart
BHV19400182-2	TRV-0003-2S	2x10^6	RFP (constitutively expressed)	GFP	CD16A-RFP/NFAT-GFP	$895	Add to Cart
BHV19400182-3	TRV-0003-3S	2x10^6	GFP (constitutively expressed)	GLuc	CD16A-GFP/NFAT-GLuc	$895	Add to Cart
BHV19400182-4	TRV-0003-4S	2x10^6	RFP (constitutively expressed)	GLuc	CD16A-RFP/NFAT-GLuc	$895	Add to Cart
BHV19400182-5	TRV-0003-5S	1x10^6	RFP (constitutively expressed)	GFP	CD16A-Puro/NFAT-GFP-GLuc	$895	Add to Cart
BHV19400182-6	TRV-0003-6S	1x10^6	Blasticidin	GFP-P2A-GLuc	CD16A-BSD/NFAT-GFP-GLuc	$895	Add to Cart
BHV19400182-7	TRV-0003-7S	1x10^6	Puromycin	RFP-P2A-GLuc	CD16A-Puro/NFAT-RFP-GLuc	$895	Add to Cart
BHV19400182-8	TRV-0003-8S	1x10^6	Blasticidin	RFP-P2A-GLuc	CD16A-BSD/NFAT-RFP-GLuc	$895	Add to Cart

Can I purchase the plasmid for this product?

TF reporter products such as this one are not available in plasmid format. They are only available as high-titer lentiviral particles. Other gene products on our site, such as ORF cDNA and shRNA products are available in either format.

CD16A/NFAT Reporter Lentivirus

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