Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3zeta)

Mfr.No.	78600
Description	The anti-CD19 CAR lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv portion of anti-CD19 (clone FMC63) linked to 2nd generation CAR (Chimeric Antigen Receptor), containing CD8 hinge, 4-1BB and CD3ζ signaling domains. The lentiviruses also contain a puromycin selection marker. The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM. Virus particles can be packaged in custom formulations by special request, for an additional fee. CD19 (also known as Cluster of Differentiation 19, B-lymphocyte surface antigen B4, or CVID3) is a glycoprotein expressed at the surface of B lymphocytes through most phases of B cell maturation. It is strictly required for B cell terminal differentiation. Mutations in the CD19 gene cause severe immune-deficiency syndromes associated with impaired antibody production such as CVID3 (common variable immuno-deficiency 3). The majority of B cell malignancies express normal to high levels of CD19, making it a nearly ideal target for cancer immunotherapy. Blinatumomab, a CD19/CD3 bi-specific T cell engager (BiTE) has been approved for relapsed/refractory B precursor ALL (Acute lymphoblastic leukemia) and CD19 was the target of the first approved CAR-T cell therapy. Studies of CD19 function and expression profiles will continue to broaden our knowledge and support broader applications in cancer therapy. Biosafety None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Species	Replication Incompetent HIV
Synonyms	Anti-CD19 Lentivirus, CAR Lentivirus, Anti-CD19 Lenti, Lenti, Lentivirus, CD19, CD8, 4-1BB, CD3ζ, Anti-CD19
Applications Range	Positive control for anti-CD19 CAR evaluation in T cells. Transduction optimization experiments. Generate anti-CD19 CAR-T cells (for research use only, not for therapeutic purposes).
Storage	Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C.
Shipping	-80°C