12 Well glass bottom plates

Mfr.No.

P12-1.5H-N, P12-1.5P

Description

Designed for high resolution imaging such as confocal microscopy.

12 well glass bottom plates are often used in experiments that need medium to large sample size.

Suitable for:

Differential Interference Contrast (DIC)
Widefield Fluorescence
Confocal Microscopy
Two-Photon and Multiphoton Microscopy
Fluorescence Recovery After Photobleaching (FRAP)
Förster Resonance Energy Transfer (FRET)
Fluorescence Lifetime Imaging Microscopy (FLIM)
Total Internal Reflection Fluorescence (TIRF)
Super-Resolution Microscopy

Recommended for:

Confocal Microscopy
Super-Resolution Microscopy

Specification

Product #	P12-1.5H-N	P12-1.5P
Frame color	black	black
Coverslip	#1.5 high performance cover glass (0.170±0.005mm)	#1.5 glass-like polymer (0.175±0.010mm)
Length	127.50 mm	127.50 mm
Width	85.35 mm	85.35 mm
Height	19.95 mm	19.95 mm
Height with lid	22.50 mm	22.50 mm
Stacking height (with lid)	19.45 mm	19.45 mm
Bottom height	0.83 mm (bottom of coverslip to plate bottom)	0.83 mm (bottom of coverslip to plate bottom)
Bottom height tolerance	±50μm (whole plate)
Well to well center distance	26.00 mm	26.00 mm
Well bottom area	382 mm2	382 mm2
Maximum volume	7.2 ml	7.2 ml
Temperature Range	-20°C to 50°C	-20°C to 50°C

Features

Suitable for long term tissue culture
Manufactured in a class 100,000 clean room
12 well plate frames are made from virgin polystyrene.
German cover glass or glass-like cover slip of superior optical quality
A USP class VI adhesive is used to assemble the cover glass and the plate.
Individually packed in easy to peel trays
Sterilized by Gamma radiation.
Conforms to ANSI/SBS 1-2004 standards

Technical Notes

Coverslip	Thickness (mm)	Material
#0	0.085-0.115	Cover glass
#1	0.13-0.16	Cover glass
#1.5	0.16-0.19	Cover glass
#1.5H	0.170±0.005	Extremely flat high peformance cover glass
#1.5P	0.175±0.01	Tissue culture treated polymer cover slip with optical characteristics very similar to that of a cover glass. Recommended for applications where better cell attachment is desired. Not suitable for TIRF. Plates with large wells, such as the 12/24 well plates, that uses #1.5P coverslip may not be suitable for applications such as whole plate auto-scan, where whole plate flatness is critical. Because the film is soft and therefore is slightly curved within large wells.
#1.5Grid	0.16-0.19	Cover glass with a 20x26 Grid

Dimension diagram (units in mm)

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Combination SKU	Supplier No.	Packing	Coverslip	Well Size	Your price	Quantity
BHI20500015-1	P12-1.5H-N	20/case	#1.5H	22.0 mm	$323.70		Add to Cart
BHI20500015-2	P12-1.5P	20/case	#1.5P	22.0 mm	$287.30		Add to Cart

12 Well glass bottom plates

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