KLH (Keyhole Limpet Hemocyanin)

Mfr.No.	MG29F-KLH
Description	KLH Coated Plates are offered with 12 breakable strips of 8 wells assembled on single well holding frame. Clear breakable strips are manufactured in pure polystyrene with low fluorescence and can be used for ELISA assays. These strips are designed to be easily breakable by the user and repositioned in the frame; the radius edged inner bottom of the wells improves the efficiency of washing. The KLH Coated 96 Well Breakable Strip Plates comply with SBS standards and the design assures a good performance in automatic processing plant. Plates are pre blocked with proteic blocking Recommended working volume of 50 to 100 μl Each coated plate is packed in a single barrier bag with desiccant Every single unit contains 5 plates White and black KLH Coated 96 Well Breakable Strip Plates can be used for Luminescence and Fluorescence assays are AVAILABLE BY ORDER ONLY. Biomat other 96 Well Plates for Immunotoxicity ELISA Tests: DNP Coated 96 Well Breakable Strip Plates Tetanus Toxoid Coated 96 Well Breakable Strip Plates TNP Coated 96 Well Breakable Strip Plates
Plate Type	Breakable Plates
Surface	KLH Coated
Technical Notes	1. Add 100 μl of different concentrations of monoclonal mouse IgG anti KLH (BioLegend code 408502) at 0.5 mg/ml), from 0.0005 to 0.500 μg/ml, diluted in Sample Diluent, (Biomat code 400-1-100) to the wells of KLH coated plate and incubate for 60 minutes at room temperature 2. Empty the wells and wash with Wash Buffer, (Biomat code 200-1-100) four times 3. Add 100 μl/well of goat anti-mouse IgG -HRP (Jackson ImmunoResearch code 115-035-003), diluted 1:25,000 in Diluent for HRP conjugate, (Biomat code 400-2-100) and incubate for 60 minutes at room temperature 4. Empty the wells and wash with Wash Buffer, (Biomat code 200-1-100) four times 5. Add 100 μl/well of TMB substrate solution, (Biomat code 500-1-100) and incubate 15 minutes at room temperature 6. Stop the substrate reaction by adding 100 μl /well of sulphuric acid, (Biomat code 600-1-100) and read the optical density values at 450 nm The data show that a plateau has got starting with a mouse IgG anti KLH concentration of 0.250 μg/ml. This concentration means the well binding capacity we can express as: - μg/well = 0.025 (25 ng/well) Figure 2: the figure gives an idea of the dilution factor to apply to the serum/plasma of the immunized mouse under evaluation; where k means a dilution of 1:1,000
Specifications	Available configurations 96-well microplates with 12 removable 8-well strips. Coating KLH is coated using 100 μl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability. Binding capacity Microplate was saturated with mouse IgG anti KLH at a concentration of 0.250 μg/ml (25 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate (see Technical notes 39 and Figure 1 and 2 for data and experiment details). The Biomat KLH microplate shows a nominal binding capacity of ~ 0.250 μg/ml of mouse IgG antiKLH Uniformity Microplates show a CV% less than 5 when used as a sandwich of mouse IgG anti KLH in an ELISA format using goat anti mouse IgG -HRP as detector and TMB as substrate. Storage and Stability The microplates, under the indicated storage conditions 2-8 °C, are stable until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Reference	KLH COATED , KLH coated surface on the KLH Coated 96 Well Breakable Strip Plates can be either used as vaccine carrier protein acting as the hapten carrier part of the vaccine component or as a highly immunogenic antigen in order to assess the immune competence of an organism and as a carrier of low molecular mass peptide and haptens, such as oligosaccharides, gangliosides or (glyco)peptides, designed to facilitate antibody production. https://www.biomat.it/wp-content/uploads/2019/12/data-sheet-KLH-coated_strip-plate.pdf